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木霉菌既是广泛应用的防治植物病害的生防菌,又是一类很有应用潜力的环境污染修复菌。针对分离筛选出的6株高效降解毒死蜱的木霉菌株,进行了土传植物真菌病害的生防活性试验。结果表明,在对峙培养条件下,供试木霉菌株对几种病原真菌均具有较为显著的抑制率,发酵滤液对多数病原真菌具有明显的抑菌作用。所有供试木霉菌株能在立枯丝核菌、灰霉、终极腐霉菌落上着生,并逐渐覆盖全部菌落;但不能在茄腐镰孢菌、尖孢镰孢菌、大丽轮枝菌上生长。真菌重寄生现象观察结果表明,供试木霉菌仅对立枯丝核菌具有明显的重寄生现象。研究结果表明,筛选出的高效降解毒死蜱的木霉菌菌株可对多种土传植物病原真菌具有良好的生防潜力。
Trichoderma is not only a widely used biocontrol agent for controlling plant diseases, but also a type of bacteria that has great potential for application in environmental remediation. Six isolates of Trichoderma strains that degrade chlorpyrifos were isolated and screened for biocontrol activity of soil-borne fungal diseases. The results showed that under confrontation culture conditions, the test Trichoderma strains against several pathogenic fungi have a significant inhibition rate, the fermentation filtrate on most pathogenic fungi have significant inhibitory effect. All Trichoderma strains tested were able to colonize Rhizoctonia solani, Botrytis cinerea, and Ultimate Pythium, and gradually covered all colonies; however, they could not be cultured in Fusarium solani, Fusarium oxysporum, Growth on bacteria. Observation of fungal re-parasitization The results showed that the tested Trichoderma only had obvious re-parasitism to Rhizoctonia solani. The results showed that Trichoderma strains screened to efficiently degrade chlorpyrifos have good biocontrol potentials against many soil-borne pathogenic fungi.