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目的通过对大鼠实验性蛛网膜下腔出血后脑血管痉挛的观察,对缺血缺氧造成的脑损伤机制进行探讨。方法用反转录聚合酶链式反应技术检测大鼠SAH后CVS时海马组织Bcl-2和BaxmRNA的表达变化。结果Bcl-2mRNA的表达水平在蛛网膜下腔组1d时即开始下降,5～7d时降至最低。BaxmRNA的表达1d呈上升趋势,5～7d时达最高。在对照组和假手术组海马组织内的Bcl-2和BaxmRNA的表达水平保持相对恒定。结论提示Bcl-2和Bax可能参与了缺血缺氧所致的神经元损伤。
Objective To investigate the mechanism of cerebral injury induced by hypoxia and hypoxia by observing cerebral vasospasm after experimental subarachnoid hemorrhage in rats. Methods The expressions of Bcl-2 and Bax mRNA in the hippocampus were detected by reverse transcription-polymerase chain reaction (RT-PCR) after CVS in rats. Results The expression of Bcl-2 mRNA began to decline in the subarachnoid group at 1 day and dropped to the lowest level at 5 to 7 days. The expression of Bax mRNA increased on the first day and reached the highest on the 5th ~ 7th day. The expression levels of Bcl-2 and Bax mRNA in the hippocampus of the control and sham groups remained relatively constant. Conclusions Bcl-2 and Bax may be involved in neuronal damage induced by ischemia and hypoxia.