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本文采取微波提取法从香蕉皮中提取得到黄酮粗提物,利用H103大孔树脂对提取物进行纯化,与丙酸复配得到复配剂。以香蕉果皮黄酮-丙酸复配剂为受试物,根据国家卫生部发布的《食品安全性毒理学评价程序和方法》对其进行毒理学试验,以便在生产实践中推广应该。利用小鼠为试验对象,进行了急性毒性试验(LD50)、骨髓细胞微核试验、小鼠精子畸形试验以及哺乳动物微粒体酶试验(Ames试验),对复配剂的毒理性进行了研究。结果表明,雌小鼠LD50为10.80 g/kg BW,可信限为7.41~15.70 g/kg BW;雄小鼠LD50为12.60 g/kg BW,可信限为7.75~20.50 g/kg BW;骨髓细胞微核试验、精子畸形试验、Ames试验的结果都为阴性,表明香蕉果皮黄酮-丙酸复配剂对小鼠体细胞和生殖细胞无诱变作用,也无直接或间接的致突变作用,属实际无毒级物质。香蕉果皮黄酮-丙酸复配剂可作为食品防腐添加剂。
In this paper, microwave extraction was used to extract the flavonoids from banana peel, and the extract was purified with H103 macroporous resin and complexed with propionic acid. Banana pericarp flavonoids - propionic acid compound as the test substance, according to the Ministry of Health issued the “Food Safety Toxicology Evaluation Program and Method” to its toxicology test, so that it should be promoted in the production practice. Acute toxicity test (LD50), micronucleus test of bone marrow cells, mouse sperm abnormality test and Ames test of mammalian mice were used to study the toxicity of the compound . The results showed that the LD50 of female mice was 10.80 g / kg BW and the confidence limit was 7.41-15.70 g / kg BW. The LD50 of male mice was 12.60 g / kg BW, with a confidence limit of 7.75-20.50 g / kg BW. Cell micronucleus test, sperm deformity test, Ames test results were negative, indicating that banana pericarp flavonoids - propionate complex on mice somatic and germ cells without mutagenic effect, nor direct or indirect mutagenic effects, Is the actual non-toxic substances. Banana peel flavonoids - propionic acid compound can be used as food preservative additives.