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米托蒽醌(MNE)为一新抗癌化疗剂,按常规可制备多层脂质体。将磷脂溶于氯仿置于50 ml圆底烧瓶中,于旋转蒸发器干燥,减压下于37℃在瓶壁形成薄膜,于涡流混合器内用超声波处理1分钟。磷酯酰胆碱(PC)及二油酸磷酸酰胆碱(DOPC)为主要脂质体成分(1.6克分子比),加入胆固醇(C)(10克分子比),使脂质体膜强化。加入0.15克分子硬脂酰胺(S)或二棕榈酸磷酸酯(DP),使脂质体分别带阳电荷或阴电荷,带阴电荷的脂质体的迁移性为1.31μm/5.4 cm,接近于人红细胞。包药脂质体与游离药物的分离采用超离心法(28×10~(-5)g),进行20分钟,用生理盐水
Mitoxantrone (MNE) is a new anticancer chemotherapeutic agent that can be used to prepare multilamellar liposomes as usual. The phospholipid was dissolved in chloroform in a 50 ml round bottom flask, dried on a rotary evaporator and a thin film was formed on the bottle wall at 37 ° C under reduced pressure and sonicated for 1 minute in a vortex mixer. Phosphatidylcholine (PC) and DOPC as main liposome components (1.6 molar ratio) were added to cholesterol (C) (10 molar ratio) to reinforce the liposome membrane . 0.15M molecular stearic amide (S) or dipalmitoyl phosphate (DP) were added to make the liposomes have a positive or negative charge, respectively. The negatively charged liposomes had a mobility of 1.31μm / 5.4cm, close to Human erythrocytes. The drug-containing liposomes and free drug separation by ultracentrifugation (28 × 10 ~ (-5) g) for 20 minutes, with saline