B淋巴细胞分化的荧光染色和免疫细胞化学染色观察(文摘)

• 作 者: 许屏 李淑莲
• 机 构: 白求恩医科大学组织胚胎教研室,白求恩医科大学组织胚胎教研室
• 出 处: 白求恩医科大学学报
• 发表日期: 1981年03期
• 关键词: 淋巴细胞分化 B淋巴细胞 荧光染色 免疫反应 细胞化学 母细胞 淋巴细胞转化 红色荧光 细胞体积 抗体产生

为进一步探讨在免疫反应过程中,B淋巴细胞分化的形态学和细胞化学变化及其与抗体产生之间的关系,作者用荧光染色法和免疫细胞化学方法对免疫的家兔膕窝淋巴结涂片进行了一系列观察。 由小淋巴细胞转化的母细胞体积增大,核圆形、位于细胞的中央,呈黄色荧光。大母细胞呈现亮桔红色荧光。以甲绿—派郎宁染色时,母细胞嗜派郎宁阳性。向浆细胞分化的各种

To further explore the relationship between the morphological and cytochemical changes of B-lymphocyte differentiation and the production of antibodies in the course of immune responses, the authors used fluorescent staining and immunocytochemistry to immunostain axillary lymph nodes in rabbits. A series of observations were made. The volume of mother cells transformed by small lymphocytes increases, and the nucleus is rounded and located in the center of the cells, showing yellow fluorescence. Large mother cells show bright orange-red fluorescence. When stained with A Green-Pranine, the mother cells were addin-positive. Differentiation to plasma cells