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目的:采用高脂饲料喂养建立SD大鼠高脂血症痰证动物模型。方法:第1次实验将30只8周龄SD雄性大鼠随机分为正常组10只及模型组20只,分别给予正常饲料和高脂饲料喂养,自由饮食及饮水,于第4、6、8周末,检测血清胆固醇(TC)、总甘油三酯(TG)、低密度脂蛋白(LDL-C)及高密度脂蛋白(HDL-C)水平,8周末采血后处死动物并取出完整肝脏,称量湿重后做HE染色病理切片。第2次实验直接将8只正常组和8只模型组大鼠分别喂养8周,8周末检测大鼠的血脂情况,并将两次实验8周末血脂水平进行比较。实验过程中对大鼠的一般情况进行观察并记录。结果:第1次实验中模型组大鼠与正常组相比,第4、6周末TC升高,差异有统计学意义(P<0.05),其余3项无统计学差异,8周末血脂四项与正常组相比均无差异,模型组肝脏病理切片有明显脂肪样变,两组主动脉切片均无明显异常;第2次实验第8周末,模型组大鼠TC、TG升高,HDL-C下降,差异有统计学意义(P<0.05),LDL-C无差异。两次实验的结果具有显著差异(P<0.05)。两次实验模型组均出现了体胖、倦怠、饮食饮水减少的情况,初步判断符合痰证的证候特点。结论:采用高脂饲料喂养SD大鼠能初步建立高脂血症痰证动物模型,但其造模时间的长短及痰证证候的判断还有待进一步探索和完善,所造动物模型的稳定性和可重复性需要进一步探究。
OBJECTIVE: To establish animal model of phlegm syndrome of hyperlipemia in SD rats by feeding with high fat diet. Methods: The first experiment 30 male Sprague-Dawley rats of 8 weeks old were randomly divided into normal group (n = 10) and model group (n = 20), fed with normal diet and high fat diet, At the end of the 8th week, serum TC, TG, LDL-C and HDL-C were measured. After 8 weeks of the experiment, the animals were sacrificed and the intact liver was removed. After weighing wet weight to do HE stained sections. In the second experiment, 8 rats in normal group and 8 model group were fed for 8 weeks respectively. The blood lipid level in rats was measured at the end of the 8th week, and the levels of blood lipid in 8 weeks were compared. During the experiment, the general situation of rats was observed and recorded. Results: In the first experiment, TC in the model group at the end of the 4th and 6th week was significantly higher than that of the normal group (P <0.05), the other 3 items had no statistical difference, Compared with the normal group, there was no difference in the model group, the pathological section of the liver of the model group had obvious steatosis, and the aorta slices of the two groups had no obvious abnormalities. At the end of the second experiment, TC, TG and HDL- C decreased, the difference was statistically significant (P <0.05), LDL-C no difference. The results of the two experiments were significantly different (P <0.05). Two experimental model group appeared body fat, fatigue, diet reduced drinking water, the initial judgment in line with the characteristics of phlegm syndromes. Conclusion: The animal model of hyperlipidemia phlegm syndrome can be established initially by feeding high fat diet to SD rats. However, the time of modeling and the judgment of phlegm syndrome remains to be further explored and perfected. The stability of animal model And repeatability needs to be explored further.