论文部分内容阅读
目的探讨在TiO_2纳米管表面固定成骨细胞特异性识别多肽的方法,为钛种植体表面生物化修饰提供新的方法和思路。方法应用多巴胺共价键合将多肽固定在TiO_2纳米管基材表面,构建生物活性涂层。结果通过场发射扫描电镜、原子力显微镜、接触角测量仪、X射线光电子能谱仪、荧光显微镜对样品进行表征,证实钛纳米管表面能够成功固定成骨细胞特异性多肽。结论通过化学偶联的方法,可将成骨细胞特异性识别多肽固定在钛纳米管基材表面,成功构建生物活性涂层。
OBJECTIVE: To investigate the method of identifying specific osteoblasts on the surface of TiO 2 nanotubes to provide new methods and ideas for the biochemical modification of titanium implants. Methods The polypeptide was immobilized on the surface of TiO 2 nanotubes by the covalent bonding of dopamine to construct bioactive coating. Results The samples were characterized by field emission scanning electron microscopy, atomic force microscopy, contact angle measurement, X-ray photoelectron spectroscopy and fluorescence microscopy. The results showed that the osteoblast-specific polypeptides could be successfully immobilized on the surface of titanium nanotubes. Conclusions The osteoblast-specific peptide can be immobilized on the surface of titanium nanotubes by chemical coupling, and the bioactive coating can be successfully constructed.