论文部分内容阅读
目的：探讨癌基因Ｎ－ｒａｓ、ｃ－ｍｙｃ及其蛋白表达与骨肉瘤的关系。方法：Ｓｏｕｔｈｅｒｎｂｌｏｔ和免疫组织化学（ＬＳＡＢ法）同步检测９例人骨肉瘤Ｎ－ｒａｓ、ｃ－ｍｙｃ癌基因和其ｐ２１ｒａｓ、ｃ－ｍｙｃ蛋白产物表达。结果：Ｎ－ｒａｓ基因有１例为缺失改变，检出率１１％（１／９），ｃ－ｍｙｃ基因有３例为扩增改变，检出率３３％（３／９）。ｐ２１ｒａｓ、ｃ－ｍｙｃ基因蛋白表达阳性率分别为７８％（７例）、８９％（８例）。ｃ－ｍｙｃ基因蛋白在骨肉瘤的表达强度为最高。在９例骨肉瘤中，两种原癌基因蛋白共同表达６例，占７０％。结论：癌基因蛋白过度表达在骨肉瘤是一个常见现象，并从癌基因和其蛋白水平上提示癌基因Ｎ－ｒａｓ、ｃ－ｍｙｃ和ｐ２１ｒａｓ、ｃ－ｍｙｃ蛋白异常改变与骨肉瘤的发生发展有关
Objective: To investigate the relationship between the expression of oncogenes N-ras, c-myc and their proteins and osteosarcoma. Methods: Southern blot and immunohistochemistry (LSAB) were used to detect the expression of N-ras and c-myc oncogenes and their p21ras and c-myc protein products in human osteosarcoma simultaneously. Results: One case of N-ras gene was deleted, the detection rate was 11% (1/9), and three cases of c-myc gene were amplified. The detection rate was 33% (3/9). The positive rates of p21ras and c-myc were 78% (7 cases) and 89% (8 cases) respectively. The expression of c-myc protein is the highest in osteosarcoma. In nine cases of osteosarcoma, the two proto-oncogene proteins co-expressed in 6 cases, accounting for 70%. CONCLUSIONS: Overexpression of oncogene proteins is a common phenomenon in osteosarcoma and it is suggested that oncogene and its protein level are related to the occurrence and development of osteosarcoma, the abnormal changes of oncogenes N-ras, c-myc, p21ras and c-myc