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目的:探讨腹腔镜和开腹手术对结直肠癌根治术患者外周血鸟苷酸环化酶C(GCC)m RNA和人端粒酶逆转录酶(h TERT)m RNA表达的影响及其意义。方法:按照随机数字表法将2009年8月-2014年4月我院收治的结直肠癌患者分为腹腔镜组和开腹手术组,通过逆转录-聚合酶链反应技术(RT-PCR)检测并比较两组患者外周血GCC m RNA和h TERT m RNA的表达情况。结果:与开腹手术组相比较,腹腔镜组患者手术时间长、术中出血量少、术后排气早、住院时间短,差异有统计学意义(P<0.01);两组患者手术前GCC m RNA和h TERT m RNA阳性率无显著差异(P>0.05);两组患者手术后GCC m RNA和h TERT m RNA阳性率显著升高,差异有统计学意义(P<0.05)。结论:腹腔镜和开腹手术均会增加结直肠癌患者血行微转移,但腹腔镜结直肠根治术不会增加术后肿瘤血循环微转移的危险性。
Objective: To investigate the effect of laparoscopic and laparotomy on expression of guanylate cyclase C (GCC) m RNA and human telomerase reverse transcriptase (h TERT) m RNA in patients with radical resection of colorectal cancer and its significance . Methods: Colorectal cancer patients admitted to our hospital from August 2009 to April 2014 were divided into laparoscopic group and laparotomy group according to random number table method. The levels of GCC m RNA and h TERT m RNA in peripheral blood were detected and compared between two groups. Results: Compared with the laparotomy group, laparoscopic group patients had longer operation time, less intraoperative blood loss, shorter postoperative exhaust and shorter hospital stay, the difference was statistically significant (P <0.01). Before operation There were no significant differences in the positive rates of Gmt m RNA and h TERT m RNA between the two groups (P> 0.05). The positive rates of Gmt m RNA and h TERT m RNA after operation were significantly higher in both groups (P <0.05). Conclusions Laparoscopy and laparotomy can increase micrometastasis in patients with colorectal cancer. However, laparoscopic colorectal surgery does not increase the risk of postoperative micrometastasis of the tumor.