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目的:探讨小檗碱对高脂-高盐-果糖诱致大鼠肾损伤保护作用的抗氧化机制。方法:取SD大鼠50只,随机分为正常对照组和造模组。造模组大鼠给予高脂-高盐饲料喂养,并交替饮用糖盐水(糖4%、盐1%)和果糖水(6%)8 w。根据血压情况,随机将高血压的动物分为模型组(10只,蒸馏水)、卡托普利(10只,25 mg/kg)组、小檗碱高剂量组(10只,300 mg/kg)和小檗碱低剂量组(10只,100 mg/kg)。继续给予高脂-高盐-果糖饲养,同时分别灌胃给予上述药物处理4 w。分别在0、4、8、10、12周末测定血压,药物处理4 w后,留取尿液测定尿蛋白含量,取血测定血肌酐和血尿素氮,分离血清测定SOD活性和MDA、GHb含量,测定肾脏组织H2O2、GSH-Px含量和CAT活性。结果:模型大鼠血压、尿蛋白、血肌酐和血尿素氮水平显著高于正常大鼠(P<0.01),并伴有血清SOD活性降低、MDA和GHb含量升高(P<0.01),肾脏组织H2O2含量升高,降低GSH-Px含量和CAT活性。小檗碱处理4 w,显著降低模型大鼠的血压、尿蛋白、血肌酐和血尿素氮水平(P<0.01),提高SOD活性,降低MDA和GHb含量(P<0.01),同时提高肾脏组织GSH-Px和CAT活性(P<0.01),轻度降低肾脏组织H2O2含量。结论:小檗碱对高脂-高盐-果糖诱致肾损伤模型大鼠具有保护作用,其作用机制可能与提高机体和肾脏组织的抗氧化能力有关。
Objective: To investigate the anti-oxidative mechanism of berberine on the protective effect of high fat-high salt-fructose on renal injury in rats. Methods: Fifty SD rats were randomly divided into normal control group and model group. Rats in model group were fed with high-fat and high-salt diet, and they were fed with sugar and salt water (sugar 4%, salt 1%) and fructose water (6%) for 8 weeks. The blood pressure, the animals were randomly divided into model group of hypertension (10, distilled water), captopril (10, 25 mg / kg) group, berberine high dose group (10, 300 mg / kg ) And berberine low dose group (10, 100 mg / kg). Continue to give high fat - high salt - fructose feeding, respectively, while gavage given the drug treatment 4 w. 0,4,8,10,12 blood pressure were measured weekend, after drug treatment 4 w, determination of urine protein Urine, blood urea nitrogen and serum creatinine was measured, assay SOD activity and MDA serum was separated, the content of GHb The contents of H2O2, GSH-Px and CAT in kidney were measured. Results: Blood pressure, urine protein, blood urea nitrogen and serum creatinine levels were significantly higher than rats with normal rats (P <0.01), and with reduced activity of SOD, MDA, GHb levels increased (P <0.01), renal Tissue H2O2 content increased, decreased GSH-Px content and CAT activity. Berberine treatment 4 w, significantly reduced blood pressure in rats with urinary protein, blood urea nitrogen and serum creatinine levels (P <0.01), to improve the activity of SOD and MDA contents GHb (P <0.01), while improving kidney GSH-Px and CAT activity (P <0.01), slightly reduce the content of H2O2 in the kidney tissue. Conclusion: berberine on high fat - high salt - fructose induced kidney injury in rats has a protective effect, the mechanism may be related to the body’s antioxidant capacity and increase kidney tissue.