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用不同特异性的黑龙江斑点热立克次体（Rickettsialheilongjiangii）054株单克隆抗体（McAb）或PCR引物检测立克次体的结果表明：群特异性的mcAb2E1和mcAb4G2用微量免疫间接荧光抗体染法（MIIF）法可检测出文中所用各种斑点热立克次体（SFGR）：立氏（R．rickettsii）、康氏（R．conerii）、小蛛（R．akari）、派克（R．parkeri）、西伯利亚（R．sibirica）、澳大利亚（R．australia）和054株立克次体；F7单抗只能检测出R．rickettsii和054株立克次体。PCR扩增结果表明：引物Rt120和Rr120．2048p—1625n分别对恙虫病立克次体（R．tsutsug－amushi）和SFGR扩增出现388bp和523bp扩增带；Rr190．70p—602n和Rr190．4442—5664n两对引物扩增结果相同，即除R．tsutsugamushi外对Q热（C．burnetii）、SFGR、普氏（R．prowazekii）和莫氏（R．mooserii）均扩增阳性，分别为532bp和1222bp电泳带；引物Rpcs．977p—1258n则可扩增上述所有立克次体，电泳带381bp。这样，可在短期内同时检测和初步鉴定不同立克次体。
Rickettsialheilongjiangii monoclonal antibody (McAb) or PCR primers were used to detect rickettsia in different specificities. The results showed that the group-specific mcAb2E1 and mcAb4G2 were detected by indirect immunofluorescence The various spotted hot rickettsia (SFGR) used in the text can be detected by the MIIF method: R. rickettsii, R. conerii, R. akari, R. parkeri ), R.sibirica, R.australia, and 054 rickettsiae; only the F7 mAb was detected. rickettsii and 054 strains of rickettsia. The results of PCR amplification showed that the amplified bands of 388bp and 523bp were amplified by Rt120 and Rr120.2048p-1625n, respectively. The amplified bands of Rt190.70p-602n and Rr190.4442 -5664n amplified the same two pairs of primers, that is, except for R. Tsutsugamushi were positive for C. burnetii, SFGR, R.prowazekii and R.mooserii, with 532bp and 1222bp bands, respectively; primer Rpcs. 977p-1258n amplifies all of the above Rickettsia, electrophoresis with 381bp. In this way, different Rickettsia species can be detected and initially identified in the short term.