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本工作用１２％乙醇麻醉的大鼠，观察了下丘脑室旁核（ＰＶＨ）微量注射Ｋ型阿片受体激动剂Ｕ－５０，４８８Ｈ对大鼠肾水钠钾排出的影响，以及第三脑室注射Ｕ－５０，４８８Ｈ对ＰＶＨ中多巴胺神经元活性的影响。结果如下：（１）ＰＶＨ微量注射Ｕ－５０，４８８Ｈ（５μｎ／ｕｌ）后２０ｍｉｎ内大鼠尿量开始增加（Ｐ＜０．０１），持续约１００ｍｉｎ，４１—６０ｍｉｎ尿量增加达峰值（Ｐ＜０．００１）。（２）ＰＶＨ预先（１０ｍｉｎ）注射Ｋ型阿片受体阻断剂ＮＢＴ（Ｎｏｒ－ＢｉｎａｌｔｏｒｐｈｉｍｉｎｅＴｅｔｒａｈｙｄｒａｔｅ）（５μｇ／ｐｌ）可以阻断Ｕ－５０，４８８Ｈ所产生的利尿效应（Ｐ＜０．０１）。（３）第三脑室注射Ｕ－５０，４８８Ｈ（１０μｇ／１０ｕｌ）２０ｍｉｎ后，ＰＶＨ中酪氨酸羟化酶免疫反应阳性（Ｔｙｒｏｓｉｎｅｈｙｄｒｏｘｙｌａｓｅ－ｉｍｍｕｎｏｒｅａｃｔｉｖｉｔｙ，ＴＨ－ＩＲ）神经元数量减少，染色强度减弱，于注药后５０ｍｉｎ变化最为显著，１００ｍｉｎ时已恢复正常。上述结果表明：ＰＶＨ微量注射Ｕ－５０，４８８Ｈ可作用于Ｋ型阿片受体引起利尿效应；第三脑室微量注射Ｕ－５０，４８８Ｈ可抑制ＰＶＨ中ＴＨ－ＩＲ神经元的免疫活性。
In this study, 12% ethanol anesthetized rats observed hypothalamic paraventricular nucleus (PVH) microinjection of K opioid receptor agonist U-50,488H rat kidney sodium and potassium efflux, and the third ventricle Effects of U-50,488H injection on dopamine neuronal activity in PVH. The results were as follows: (1) Urine volume began to increase (P <0.01) within 20min after PVH microinjection of U-50,488H (5μn / ul) <0.001). (2) Pretreatment with PVH (10 min) could block the diuretic effect of U-50,488H (P <0.01) by injecting Nor-Binaltorphimine Tetrahydrate (5μg / pl). (3) Tyrosine hydroxylase-immunoreactivity (TH-IR) neurons in PVH decreased after 20min injection of U-50,488H (10μg / 10ul) into the third ventricle, 50min after injection the most significant change, 100min has returned to normal. The above results showed that microinjection of U-50,488H into PVH induced diuretic effect induced by PVH; and microinjection of U-50,488H into the third ventricle inhibited the immunosuppressive activity of TH-IR neurons in PVH.