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以大鼠cDNA为探针，用同源方法克隆人类K-鸦片受体基因（OpioidreceptorKappa，OPRK1），对克隆片段进行测序分析发现：人的K-鸦片受体的多肽系列与大鼠的相关的跨膜构域区的Ⅲ、Ⅳ位点的多肽系列的同源性高达98.6％。而这种保守性在跨膜构域的Ⅳ、Ⅴ区之间的细胞外环上第七个氨基酸残基之后明显下降，萤光标记人k-受体的2个噬菌体克隆片段，并以此标记探针与人中期染色体进行原位杂交，发现OPRK1基因被定位于8q11．2－12区域。计数细胞染色体时没有发现有其它位置的杂交点，因此认为：在人类基因组中，仅存在一个k-鸦片受体基因。
Using rat cDNA as a probe, the human K opioid receptor gene (OPRK1) was cloned by homologous method. Sequencing analysis of the cloned fragments revealed that the human K-opioid receptor polypeptide sequence is related to the rat The homology of the polypeptide series in the Ⅲ and Ⅳ sites in the transmembrane domain was as high as 98.6%. This conservatism was significantly decreased after the seventh amino acid residue in the extracellular loop between the IV and V regions of the transmembrane domain and fluorescently labeled two phage clone fragments of the human kappa receptor In situ hybridization of the labeled probe to human metaphase chromosomes revealed that the OPRK1 gene was located in the 8q11.2-12 region. When counting chromosomes of cells, no other hybridization sites were found, so that there is only one k-opioid receptor gene in the human genome.