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目的研究人参茎叶皂甙（ＧＳＬ）提高创伤小鼠Ｔ细胞功能的分子机制。方法利用闭合性创伤小鼠模型，观察ＧＳＬ在体内外对创伤小鼠活化的Ｔ细胞白细胞介素２（ＩＬ－２）、ＩＬ－２受体α链（ＩＬ－２Ｒα）基因转录水平，ｃＡＭＰ、ｃＧＭＰ含量以及磷脂酰肌醇代谢的调节作用。结果ＧＳＬ体内应用（５０ｍｇ·ｋｇ－１·ｄ－１×４ｄ）可明显逆转创伤小鼠活化的Ｔ细胞ＩＬ－２ｍＲＮＡ、ＩＬ－２ＲαｍＲＮＡ、ＩＬ－２及ＩＬ－２Ｒα的受抑状态，降低细胞内ｃＡＭＰ含量，增加ｃＧＭＰ与三磷酸肌醇（ＩＰ３）含量，升高激离钙［Ｃａ２＋］ｉ）浓度、钙调素（ＣａＭ）、ＣａＭ依赖的蛋白激酶（ＣａＭ－ＰＫ）及蛋白激酶Ｃ（ＰＫＣ）的活性。０．１～１００μｇ／ｍｌ的ＧＳＬ在体外可升高创伤小鼠活化的Ｔ细胞ＩＬ－２ｍＲＮＡ及ＩＬ－２ＲαｍＲＮＡ水平，降低ｃＡＭＰ含量，升高ｃＧＭＰ含量、［Ｃａ２＋］ｉ浓度及ＰＫＣ活性。结论ＧＳＬ可通过调节Ｔ细胞内环核苷酸含量及促进磷脂酰肌醇代谢，进而增强创伤后活化的Ｔ细胞内ＩＬ－２及ＩＬ－２Ｒα的基因转录表达
Objective To study the molecular mechanism of Ginseng stem and leaf saponin (GSL) in enhancing T cell function in traumatic mice. METHODS: A closed wound mouse model was used to investigate the transcriptional levels of interleukin-2 (IL-2) and interleukin-2 receptor alpha (IL-2Rα) genes in activated T cells of traumatic mice induced by GSL in vitro and in vivo. cGMP content and regulation of phosphatidylinositol metabolism. Results The in vivo application of GSL (50 mg·kg-1·d-1×4d) significantly reversed the inhibitory state of IL-2 mRNA, IL-2Rα mRNA, IL-2, and IL-2Rα in activated T cells of traumatic mice and decreased intracellular cAMP content increased cGMP and inositol trisphosphate (IP3) levels, increased concentration of exogenous calcium [Ca2+]i, calmodulin (CaM), CaM-dependent protein kinase (CaM-PK), and protein kinase C (PKC) ) activity. 0.1-100 μg/ml of GSL could increase the mRNA expression of IL-2 mRNA and IL-2Rα, decrease the content of cAMP, and increase the content of cGMP, [Ca2+]i and PKC in activated T cells of traumatic mice. Conclusions GSL can increase the transcription and expression of IL-2 and IL-2Rα in activated T cells by regulating the content of cyclic nucleotides in T cells and promoting the metabolism of phosphatidylinositol.