论文部分内容阅读
目的 研究儿童过敏性哮喘与血清总 Ig E、尘螨特异性 Ig E、尘螨过敏原皮肤点刺试验的关系。方法 采用双抗体夹心酶联免疫吸附试验 (EL ISA) ,检测了 87例哮喘患儿血清总 Ig E和尘螨特异性 Ig E水平 ,应用国际标准方法做尘螨过敏原皮肤点刺试验 ,并比较了三种检查结果之间的关系。结果 哮喘患儿的血清总 Ig E为 (5 42 .84± 32 1.85 ) IU / m l,尘螨特异性Ig E阳性率为 71.3% ,尘螨过敏原皮肤点刺试验阳性率 83.9% ,正常对照组 42名健康儿童总 Ig E(2 2 1.76± 2 13.73) IU / ml,尘螨特异性 Ig E阳性率 19.0 % ,皮肤点刺试验阳性率 2 3.8% ,三种试验两组间统计学处理有极显著差异 (P<0 .0 1) ;血清总 Ig E增高 (>45 0 IU/ ml)与尘螨特异性 Ig E阳性符合率 91.5 % ;血清尘螨特异性 Ig E阳性与皮肤点刺试验阳性符合率为 75 .6 %。结论 哮喘儿童血清总 Ig E水平、尘螨特异性 Ig E阳性率及尘螨皮肤点刺试验阳性率均明显高于健康儿童。
Objective To study the relationship between childhood allergic asthma and serum total Ig E, dust mite-specific Ig E, and dust mite allergen skin prick test. Methods Serum total Ig E and dust mite specific Ig E levels were measured in 87 asthmatic children by double antibody sandwich enzyme-linked immunosorbent assay (ELISA). Skin dust prick tests were conducted using the international standard method The relationship between the three test results is compared. Results The total serum IgE of asthmatic children was (5 42.84 ± 32 1.85) IU / ml, the positive rate of dust mite-specific Ig E was 71.3%, and the positive rate of dust mite allergen skin prick test was 83.9% In the 42 healthy children, the total Ig E (2 2 1.76 ± 2 13.73) IU / ml, dust mite-specific Ig E positive rate was 19.0%, and the skin prick test positive rate was 3.8%. There were statistically significant differences between the three groups (P <0.01). The positive rate of total Ig E in serum (> 45 0 IU / ml) and dust mite-specific Ig E were 91.5% Stab test positive coincidence rate of 75.6%. Conclusions Serum total Ig E levels, dust mite-specific Ig E positive rates, and dust mite skin prick tests were significantly higher in asthmatic children than those in healthy children.