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本文用NMR和生化方法研究了竹红菌乙素对人红细胞膜Na~+-K~+ATPase和钠通透性的光敏损伤。结果表明:在通常情况下,可同时观察到乙素对Na~+-K~+ATPase和钠通透性的光敏损伤。比较乙素、甲素、原卟啉和胆红素对上述两项指标的光敏能力,发现乙素对Na~+-K~+ATPase损伤能力与甲素和原卟啉相当,比胆红素大,对钠通透性的损伤大于其它几种敏化剂。实验指出,Na~+-K~+ATPase活力下降比钠通透性增加敏感。在乙素光敏作用时,加入Vit E可基术上保持胞内钠离子浓度不变,但无法使Na~+-K~+ATPase活力不损伤,这表明膜磷脂的结构完整对保持胞内钠浓度比较重要。对照试验指出乙素可使Na~+-K~+ATPase暗失活,这可能是经乙素介导的,由膜还原物质向氧的电子传递生成活性氧自由基攻击靶分子所致。
In this paper, the photochemical damage of Hypocrellin B to human erythrocyte membrane Na ~ + -K ~ + ATPase and sodium permeability was studied by NMR and biochemical methods. The results showed that under normal conditions, photochemical damage of Na ~ + -K ~ + ATPase and sodium permeability could be observed simultaneously. Compared with the photosensitizing ability of B, A, P and B, we found that the damage ability of B on Na ~ + -K ~ + ATPase is comparable to A and P, Large damage to sodium permeability is greater than several other sensitizers. Experiments show that Na ~ + -K ~ + ATPase activity decline than sodium permeability sensitive. In the photosensitizing effect of B, the addition of Vit E can maintain the intracellular sodium ion concentration unchanged, but not the Na ~ + -K ~ + ATPase activity does not damage, indicating that the structural integrity of membrane phospholipids to maintain intracellular sodium Concentration is more important. Control experiments showed that B could darken the Na ~ + -K ~ + ATPase, which may be mediated by B, which is caused by the electron transfer of the membrane-reducing substance to oxygen to generate reactive oxygen radicals attacking the target molecule.