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丹那唑是一种化学上与17α-乙基睾酮相关的合成甾体,由于它具有多种内分泌特性而深受关注.自1971年始用丹那唑治疗子宫内膜异位症以来,现已阐明其药理机制至少包括四方面:①直接抑制GnRH及促性腺激素分泌;②与细胞内雄激素及孕酮受体相互作用;③直接抑制甾体生成;④改变内源性甾体的代谢.作者曾使用垂体细胞培养,证明丹那唑可以直接抑制由GnRH引起的LH释放.本文进而研究丹那唑的直接抗促性腺激素活性是否与改变垂体前叶的GnRH受体数量有关.选用21天的雌性大鼠,按照Vale等的方法稍加改良制备垂体前叶单层细胞培养.在两天的预培
Danazol, a synthetic steroid chemically related to 17α-ethyltestosterone, is of great concern because of its multiple endocrine properties Since the beginning of the treatment of endometriosis with Danazol in 1971, it has now been clarified that Pharmacological mechanisms include at least four aspects: ① direct inhibition of GnRH and gonadotropin secretion; ② intracellular androgen and progesterone receptor interactions; ③ direct inhibition of steroidogenesis; ④ endogenous steroid metabolism. The use of pituitary cell culture to prove that the direct inhibition of danazol by GnRH release of LH.This paper further study the direct anti-gonadotropin activity of danazol and change in the anterior pituitary GnRH receptor related to the number of 21-day female rats , Modified slightly according to Vale et al. To prepare monolayers of anterior pituitary cells cultured in two days of pre-culture