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目的建立小儿清肺化痰颗粒中盐酸麻黄碱和盐酸伪麻黄碱的含量测定方法。方法采用HPLC,以Phe-nomenex Luna C18为色谱柱,乙腈-0.1%磷酸(4:96)为流动相,检测波长205nm。结果试验表明,盐酸麻黄碱在0.02010～1.005μg(R=1.0000),盐酸伪麻黄碱在0.003813～0.19065μg(R=0.9998)的范围内呈线性关系,平均回收率为97.1%(RSD=1.9%)。结论本方法简便、准确,可作为该制剂的质控标准。
Objective To establish a method for the determination of ephedrine hydrochloride and pseudoephedrine hydrochloride in Pediatric Qingfei Huayu Granules. Methods HPLC was used with Phe-nomenex Luna C18 as the mobile phase and acetonitrile-0.1% phosphoric acid (4:96) as the mobile phase. The detection wavelength was 205 nm. The results showed that the ephedrine hydrochloride in the 0.02010 ~ 1.005μg (R = 1.0000), pseudoephedrine hydrochloride in the range of 0.003813 ~ 0.19065μg (R = 0.9998) a linear relationship, the average recovery was 97.1% (RSD = 1.9%). Conclusion This method is simple and accurate and can be used as the quality control standard of the preparation.