CLONING OF cDNA CODING FOR CARBAMYL PHOSPHATE SYNTHETASE I AND CHANGES IN LEVELS OF CPS_1 mRNA DURIN

• 作 者: 吴石君 李士谔 张海澜 罗坚 于跃
• 机 构: Faculty of Basic Medical Sciences, Peking Union Medical College, Institute of Basic Medical Sciences
• 出 处: Science in China,Ser.B
• 发表日期: 1988年02期
• 关键词: plasmid carcinogenesis malignancy insert screening crease synthetase length clon

cDNA coding for carbamyl phosphate synthetase I was cloned from recombinant plasmid with insertcomplementary to the mRNA for CPS_1 followed by hybrid-selected translation screening. The length ofthe insert CPS_1 cDNA was approximately 800 base pairs. Using this cDNA as a probe, it was foundby dot-blot analysis of the total RNAs and poly(A)~+-RNAs isolated from rat livers with different path-ological lesions induced by diethylnitrosamine that the levels of CPS_1 mRNA were decreased, the de-crease being correlated with the malignancy of hepatocytes during carcinogenesis.

cDNA coding for carbamyl phosphate synthetase I was cloned from recombinant plasmid with insertcomplementary to the mRNA for CPS_1 followed by hybrid-selected translation screening. The length of the insert CPS_1 cDNA was approximately 800 base pairs. Using this cDNA as a probe, it was foundby dot -blot analysis of the total RNAs and poly (A) ~ + -RNAs isolated from rat livers with different path-ological lesions induced by diethylnitrosamine that the levels of CPS_1 mRNA were decreased, the de-crease being correlated with the malignancy of hepatocytes during carcinogenesis.