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我们于1990年5～6月份对卫生室医疗器械HBsAg污染情况进行了调查。现报告如下: 1.研究对象随机抽取7处乡镇的40个卫生室使用的医疗器械。将调查对象分为3类:①来消毒医疗器械:镊子、体温表、血压计、听诊器等;②消毒医疗器械:消毒后的针筒、针头、煮沸器等;③环境物品:桌子、凳子等。 2.实验方法 (1)采样方法:无菌试管内放入2ml无菌pH7.4P.B.S液,以无菌棉拭子蘸取P.B.S液,在被检器物表面反复擦拭,然后放回原试管内,置4℃过夜,拧出液体,3000rpm离心30分钟,取上清液检验。 (2)检验方法:用反向间接血凝法(RPHA)检测HBsAg,按《全国病毒性肝炎检测规程》,阳性者均用中和试验证实。HBsAg诊断血球由上海
We conducted a survey of HBsAg contamination in medical clinics from May to June 1990. The report is as follows: 1. Subjects randomly selected medical devices used in 40 clinics in 7 townships. The survey is divided into three categories: ① to disinfect medical equipment: tweezers, thermometer, sphygmomanometer, stethoscope, etc .; ② disinfection of medical equipment: sterile syringes, needles, boilers, etc .; ③ environmental items: tables, stools, etc. . 2. Experimental Methods (1) Sampling Method: Sterile test tube placed 2ml sterile pH7.4P.BS solution to a sterile cotton swab dipped in PBS solution, repeatedly rubbed on the surface of the test object, and then back to the original tube Inside, set overnight at 4 ℃, unscrew the liquid, centrifuged at 3000rpm for 30 minutes, the supernatant test. (2) Test Method: HBsAg is detected by reverse indirect hemagglutination (RPHA). According to the “National Viral Hepatitis Testing Protocol”, all positives are confirmed by neutralization test. HBsAg diagnosis of blood cells by Shanghai