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目的：为了改进放射免疫显像（ＲＩＩ），进行９９ｍＴｃ标记卵巢癌单克隆抗体ＣＯＣ１８３Ｂ２Ｆ（ａｂ’）２ＲＩＩ的研究。方法：采用无花果酶制备单抗ＣＯＣ１８３Ｂ２片段Ｆ（ａｂ’）２，改进ＳｎＣｌ２法进行９９ｍＴｃ标记片段Ｆ（ａｂ’）２及正常鼠ＩｇＧ（ＮＭＩｇＧ）；９９ｍＴｃＣＯＣ１８３Ｂ２或９９ｍＴｃＮＭＩｇＧ标记物分别经腹腔注入荷人卵巢癌裸鼠体内，１８ｈ进行ＲＩ。显像后测定并计算肿瘤与非肿瘤组织放射性活度比值（Ｔ／ＮＴ）。结果：（１）无花果酶可制备ＣＯＣ１８３Ｂ２单抗片段Ｆ（ａｂ’）２；（２）改进ＳｎＣｌ２法进行９９ｍＴｃ标记Ｆ（ａｂ’）２，标记率达９０％以上；（３）１８ｈ后ＲＩ，可见９９ｍＴｃＣＯＣ１８３Ｂ２组荷瘤裸鼠较９９ｍＴｃＮＭＩｇＧ对照组移植瘤图像清晰，且Ｔ／ＮＴ值明显高。结论：应用片段Ｆ（ａｂ’）２可改善ＲＩＩ。
OBJECTIVE: To improve radioimmunoimaging (RII), 99m Tc-labeled ovarian cancer monoclonal antibody COC183B2 F (ab ’) 2RII was studied. Methods: F (ab ’) 2 of COC183B2 fragment was prepared by flow cytometase, 99mTc labeled fragment F (ab’) 2 and normal mouse IgG (NMIgG) were modified by SnCl2 method. 99mTcCOC183B2 or 99mTcNMIgG markers were respectively injected into abdominal cavity Injected into human ovarian cancer nude mice, 18h RI. After imaging, the ratio of tumor activity to non-tumor activity (T / NT) was measured and calculated. Results: (1) F (ab ’) 2 of COC183B2 monoclonal antibody could be prepared by the method of flow cytometry; (2) 99mTc labeled F (ab’) 2 was modified by SnCl2 method and the labeling rate was over 90%; (3) 99mTc COC183B2 group of tumor-bearing nude mice can be seen 99mTc NMIgG control group of clear xenografts, and T / NT values were significantly higher. Conclusion: FII can improve RII by using fragment F (ab ’) 2.