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根据1995 ~1996 田间调查表明,CMV 是侵染白肋烟的主要病毒之一。采集到一种( 命名为CMV- TB) 引起烟株花叶、植株矮化的样品,摩擦接种能侵染7 科29 种植物。CMV- TB 引起苋色藜局部枯死斑。CMV- TB 体外稳定性状:体外保毒期2 天;稀释限点10 - 1 ;致死温度50 - 55 ℃。电镜观察CMV- TB 为球状颗粒,直径30nm 。SDS聚丙酰胺电泳测其分子量28 800D。CMV- TB 和CMV- CA( 花生株系) 抗血清起强阳性反应,与同一病毒组的花生矮化病毒(PSV) 抗血清起阴性反应。基于以上性质,CMV- TB 经鉴定属于黄瓜花叶病毒。本研究为侵染白肋烟的CMV 国内首次详细报道。通过应用生物学( 寄主反应和温度敏感性) 实验与CMV- S、CMV- D 对比,参试的分离物在32 ℃下引起昆诺藜( C.qinoa) 、豇豆(V.siesis) 产生局部枯斑并且与CMV- D 抗血清产生比CMV- S抗血清更强的特异性血清学反应。以上实验证明,CMV- TB 中国分离物与CMV- D 性质相同,属于CMV 亚组I,而与CMV 亚组II有明显差异。依据在一组寄主植物上的反应,对参试的CMV- TB 进行了株系划分
According to field surveys from 1995 to 1996, CMV is one of the major viruses that infect Burley tobacco. A sample (named CMV-TB) that caused the dwarfing of tobacco mosaic and plants was collected. Frictional inoculation could infect 29 species belonging to 7 families. CMV-TB caused Amaranthus currant local dead spots. CMV-TB in vitro stability traits: 2 days in vitro protection period; dilution limit 10 - 1; lethal temperature 50 - 55 ℃. Electron microscopy CMV-TB spherical particles, a diameter of 30nm. SDS polyacrylamide electrophoresis measured molecular weight of 28 800D. CMV-TB and CMV-CA (peanut strain) antiserum reacted strongly with antisera to peanut dwarf virus (PSV) in the same virus group. Based on the above properties, CMV-TB was identified as cucumber mosaic virus. This study reports the first CMV infection in Burley. The tested isolates caused C. quinoa and V.siesis to produce topicality at 32 [deg.] C by application of the biology (host reaction and temperature sensitivity) experiment to CMV-S, CMV-D Blot spots and stronger specific serological responses to CMV-D antisera than CMV-S antisera. The above experiments show that, CMV-TB Chinese isolates with the same nature as CMV-D, belonging to the CMV subgroup I, and CMV subgroup II have significant differences. Based on the responses on a group of host plants, the tested CMV-TB lines were divided