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目的 分析鼻咽癌 (NPC) 13号染色体长臂 (13q)和 14号染色体长臂 (14q)上 2 1个位点的等位基因杂合子丢失(LOH) ,并分析这些位点的LOH与NPC临床病理及EBV感染的关系。方法 用聚合酶链反应 (PCR)为基础的微卫星多态性分析技术结合基因扫描和基因绘图技术对 6 0例NPC进行LOH分析。结果 13q染色体发生一个或多个位点LOH频率为 78% ,高频率LOH(大于 30 % )位点集中于 13q12 3 q14 3和13q32附近。 14q染色体发生至少一个位点LOH的频率为 80 % ,高频率丢失位点集中于 14q11 q13、14q2 1 q2 4和14q32附近。 13q31 q32位点的LOH与低滴度血清EBVEA/IgA有关 ;14q染色体的LOH与NPC细胞的分化差有关。结论 华南地区鼻咽癌在 13q和 14q染色体发生高频率的LOH ,这些缺失区可能存在多个在NPC发生发展过程中起重要作用的肿瘤抑制基因。
Objective To analyze allele heterozygosity loss (LOH) at 21 loci on the long arm of chromosome 13 (13q) and 14q of chromosome 14 in nasopharyngeal carcinoma (NPC) and to analyze the relationship between LOH and Relationship between clinical pathology and EBV infection in NPC. Methods Polymerase chain reaction (PCR) -based microsatellite polymorphism (PCR) technique was used to analyze LOH of 60 NPCs by combining gene scanning and gene mapping techniques. RESULTS: One or more loci on chromosome 13q had a LOH frequency of 78%, while high frequency LOH (> 30%) focused on 13q12 3 q14 3 and 13q32. Occurrence frequency of at least one locus LOH on chromosome 14q is 80%, and high frequency loss sites are concentrated on the vicinity of 14q11 q13, 14q21 q2 4 and 14q32. LOH at 13q31 q32 locus was associated with low titer serum EBVEA / IgA; LOH at chromosome 14q was associated with poorly differentiated NPC cells. Conclusions High frequency LOH occurs in the 13q and 14q chromosomes of nasopharyngeal carcinoma in southern China. There may exist multiple tumor suppressor genes that play an important role in the development of NPC in these missing regions.