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目的:探讨α-细辛醚通过bcl-2途径对人肺癌细胞系A549增殖抑制作用及凋亡诱导作用。方法:人肺癌A549细胞孵育24 h后,分别加入不同剂量α-细辛醚(0.25、0.5、1 mg/ml)作用于A549细胞,继续培养12,24,36,48 h,采用MTT法检测不同浓度α-细辛醚对A549细胞的增殖抑制情况;流式细胞术检测细胞凋亡;实时荧光定量PCR法检测凋亡相关基因bcl-2和bax mRNA的表达,western blot检测bcl-2和bax蛋白表达水平。结果:随着α-细辛醚剂量(0.25、0.5、1 mg/ml)的增加,可明显抑制A549细胞的增殖,这种抑制作用具有时间和剂量依赖性;流式细胞仪检测细胞发生了凋亡;α-细辛醚不同剂量(0.25、0.5、1 mg/ml)作用48h后bcl-2 mRNA和蛋白表达明显减少,bax mRNA和蛋白表达明显增加。结论:α-细辛醚对人肺癌A549细胞有明显的增殖抑制作用,并呈现时间和剂量依赖性;α-细辛醚可通过下调bcl-2表达和上调bax表达而诱导人肺癌A549细胞发生凋亡。
AIM: To investigate the inhibitory effect of α-asarone on human lung cancer cell line A549 and the induction of apoptosis by bcl-2 pathway. Methods: Human lung adenocarcinoma A549 cells were incubated for 24 hours, and A549 cells were treated with different dosages of α-asarone (0.25, 0.5, 1 mg / ml) respectively. The cells were cultured for 12,24,36,48 h. MTT assay The inhibitory effects of different concentrations of α-asarone on the proliferation of A549 cells were detected by flow cytometry. The expressions of bcl-2 and bax mRNA were detected by real-time fluorescence quantitative PCR. The expressions of bcl-2 and bcl- bax protein expression level. Results: The proliferation of A549 cells was significantly inhibited by the dose of α-asarone (0.25, 0.5, 1 mg / ml), and the inhibition was time-and dose-dependent. The cells were detected by flow cytometry The mRNA and protein expressions of bcl-2 and bax mRNA and protein were significantly increased after a dose of 0.25, 0.5 and 1 mg / ml of α-asarone for 48 h. CONCLUSION: α-asarone can significantly inhibit the proliferation of human lung cancer A549 cells in a time and dose-dependent manner. Α-asarone can induce human lung adenocarcinoma A549 cells by down-regulating the expression of bcl-2 and up-regulating the expression of bax Apoptosis.