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为研究ＡｐｏＥ基因对中国人非胰岛素依赖型糖尿病（ＮＩＤＤＭ）患者血脂水平的影响，应用聚合酶链反应（ＰＣＲ）扩增出ＡｐｏＥ基因第４外显子内的２９２ｂｐ片段，继以限制性内切酶ＨｈａⅠ酶解消化，最后行８％聚丙烯酰胺凝胶电泳，根据电泳带型判断ＡｐｏＥ基因型。共检测了１１２例非胰岛素依赖型糖尿病患者的ＡｐｏＥ基因型。通过比较不同ＡｐｏＥ基因型与血脂水平的关系发现：ＡｐｏＥ基因多态性与ＴＧ，ＨＤＬ－Ｃ，ＡｐｏＡⅠ，ＡｐｏＢ及Ｌｐ（ａ）水平无相关性，而与ＴＣ（Ｐ＝０．００２９）及ＬＤＬ－Ｃ（Ｐ＝０．００２１）水平相关，携带ε４等位基因者具有较高的ＴＣ及ＬＤＬ－Ｃ水平，而携带ε２等位基因者具有较低的ＴＣ及ＬＤＬ－Ｃ水平。
In order to study the effect of ApoE gene on blood lipid levels in Chinese patients with non-insulin dependent diabetes mellitus (NIDDM), a 292 bp fragment of ApoE gene exon 4 was amplified by polymerase chain reaction (PCR), followed by restriction endonuclease Enzyme Hha Ⅰ digestion, the final line of 8% polyacrylamide gel electrophoresis, ApoE genotypes according to the electrophoresis band. ApoE genotypes were detected in 112 non-insulin-dependent diabetic patients. By comparing the relationship between different ApoE genotypes and blood lipid levels, it was found that ApoE gene polymorphisms were not associated with TG, HDL-C, ApoAⅠ, ApoB and Lp (a) levels, but not with TC (P = 0.0029) C (P = 0.0021). The level of TC and LDL-C were higher in those with ε4 allele, while those with ε2 allele had lower TC and LDL-C levels.