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1 材料和方法1.1 材料活检标本取自260例患者,年龄18岁～74岁,平均40.3岁,男女比例为1.36:1,其中内镜诊断为慢性胃炎194例,胃溃疡14例,十二指肠球部溃疡52例.所有患者均未接受抗 Hp 治疗,胃癌和胃结核未纳入本研究.抗 HLA-DR 单克隆抗体为丹麦 Dako 公司产品,ABC 试剂盒为美国 Vector 公司产品.1.2 方法每例患者均在胃窦采集胃粘膜组织3块,1块用作Hp 培养,1块用作快速尿素酶实验,另1块作病理学检查.快速尿素酶检查或 Hp 培养出现一项阳性即认为有 Hp 感染存在.免疫组化染色采用 ABC 过氧化物酶法.用正常鼠血清代替一抗进行染色,作为阴性对照.用磷酸缓冲液代替一抗进行染色,作为空白对照.胃粘膜组织活检标本经福尔马林固定、脱水后石蜡包埋,4μm厚连续切片,分别作 HE 染色和 ABC 免疫组化染色,应用普通光学显微镜观察,若观察到淋巴细胞聚集和清晰的生发中心即认为有淋巴滤泡的存在,用 x~2检验判定 Hp阳性组与 Hp 阴性组胃粘膜淋巴滤泡发生差异有无显著性.根据淋巴滤泡周围 HLA-DR 抗原阳性上皮细胞表达范围分别判定:无胃粘膜上皮细胞表达 HLA-DR 抗原(-),紧邻淋巴滤泡不超过一个腺体范围的上皮细胞表达 HLA-DR 抗原(+),距淋
Materials and methods 1.1 Materials Biopsy specimens taken from 260 patients aged 18 years old to 74 years old with an average of 40.3 years old, the ratio of male to female 1.36: 1, of which 194 cases of endoscopic diagnosis of chronic gastritis, gastric ulcer in 14 cases, Intestinal ulcer in 52 cases.All patients were not treated with anti-Hp, gastric cancer and gastric tuberculosis are not included in this study.Anti-HLA-DR monoclonal antibody for the Danish company Dako, ABC kit for the United States Vector Company products .1.2 Methods The patients were collected gastric mucosa in the antrum 3, 1 for Hp culture, one for rapid urease test, the other for pathological examination. Rapid urease test or Hp culture appeared a positive that Hp infection exists.Immunohistochemical staining using ABC peroxidase method with normal rat serum instead of primary antibody staining as a negative control with phosphate buffer instead of primary antibody staining as a blank control gastric mucosal biopsy specimens After formalin fixation, dehydration, paraffin-embedded, 4μm thick serial sections, respectively, HE staining and ABC immunohistochemical staining, the use of ordinary light microscopy, if the observed lymphocyte aggregation and clear germinal center that The presence of lymphoid follicles, x ~ 2 test to determine Hp positive group and Hp negative group gastric mucosal lymphoid follicles were significantly different.According to the lymphatic follicles around HLA-DR antigen-positive epithelial cell expression range were determined: no stomach The mucosal epithelial cells express HLA-DR antigen (-), HLA-DR antigen (+) is expressed in epithelial cells immediately adjacent to the lymphoid follicles in a range of one gland,