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目的:建立同时测定大鼠组织中丹皮酚和大黄酸含量的高效液相色谱法。方法:采用Phenomsil BDS C18(4.6 mm×250 mm,5μm),以乙腈-0.1%磷酸(37∶63)为流动相,流速1.0 m L·min-1,以橙黄决明素为内标,在274 nm下进行检测。结果:丹皮酚的线性范围为Y=-0.055+0.03X(r=0.993 2),日内和日间的RSD均<4.1%;大黄酸的线性范围为Y=-0.152+0.014X(r=0.993 5),日内和日间的RSD均<4.9%。组织样品的稳定性符合要求。结论:所建立的大鼠组织中丹皮酚和大黄酸HPLC测定方法,适用于大鼠体内赤芍、大黄药对的组织分布研究。
Objective: To establish a simultaneous determination of paeonol and rhein content in rat tissue by high performance liquid chromatography. METHODS: Phenolilil BDS C18 (4.6 mm × 250 mm, 5 μm) was used as a mobile phase with a mobile phase of acetonitrile-0.1% phosphoric acid (37:63) at a flow rate of 1.0 mL · min- 274 nm for testing. Results: The linear range of paeonol was Y = -0.055 + 0.03X (r = 0.993 2), with RSDs both intra-day and inter-day <4.1%. The linear range of rhein was Y = -0.152 + 0.014X (r = 0.993 5). RSDs were both lower than 4.9% during the day and during the day. The stability of the tissue sample meets the requirements. Conclusion: The established method for the determination of paeonol and rhein in rat tissue is suitable for the tissue distribution of Radix Paeoniae Rubra and Rhubarb in rats.