论文部分内容阅读
目的 :观察来昔帕泛对急性重症胰腺炎大鼠细菌移位的作用。方法 :取SD大鼠 ,分成 3组 (假手术组、对照组、治疗组 ) ,每组各 15只。术前用荧光素标记大肠杆菌给鼠灌胃后 ,对照组、治疗组制成急性胰腺炎模型。治疗组术后 15min、12h静脉注射来昔帕泛 ,对照组术后 3 0min腹腔内注射二甲亚砜。术后动态观察血小板活化因子 (PAF)的变化。术后 2 4h活杀取肠系膜淋巴结和肝制成为 10 %组织均浆 ,在荧光显微镜下细菌记数。同时光镜下观察小肠和肝病理形态变化。结果 :治疗组各细菌移位数较对照组均显著减少 ( P <0 0 1)。肠黏膜和肝病理损害也明显较轻。治疗组PAF也显著降低 ( P <0 0 1)。结论 :来昔帕泛可以减少急性胰腺炎时肠道细菌向肠系膜淋巴结和远位器官的移位 ,并对肠黏膜屏障和肝等组织器官有保护作用。PAF参与了急性胰腺炎时组织损害和细菌移位的过程
OBJECTIVE: To observe the effect of leuproprazole on bacterial translocation in rats with acute severe pancreatitis. Methods: SD rats were divided into 3 groups (sham operation group, control group and treatment group), 15 rats in each group. Preoperative fluorescein-labeled E. coli to mice after gavage, the control group, the treatment group made of acute pancreatitis model. The treatment group received intravenous injection of prostaprazole at 15 min and 12 h after operation, and dimethylsulfoxide was injected intraperitoneally at 30 min in the control group. Postoperative dynamic changes of platelet activating factor (PAF) were observed. 24 h after surgery to kill mesenteric lymph nodes and liver made of 10% tissue homogenate, under a fluorescent microscope bacterial count. At the same time, pathological changes of small intestine and liver were observed under light microscope. Results: The number of bacterial translocation in treatment group was significantly lower than that in control group (P <0.01). Intestinal mucosa and liver pathology damage is also significantly lighter. PAF in the treatment group was also significantly lower (P <0.01). CONCLUSION: Laibai Pan can reduce intestinal bacterial translocation to mesenteric lymph nodes and distant organs in acute pancreatitis and protect intestinal mucosal barrier and liver and other tissues and organs. PAF is involved in the process of tissue damage and bacterial translocation in acute pancreatitis