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目的应用组织学、免疫组化和巢式聚合酶链反应技术(PCR)对早期自然流产胎盘绒毛组织进行人微小病毒(Human parvovirus B19,简称B19)检测,探讨B19感染与早期自然流产的关系。方法采用常规HE染色、免疫组织化学和巢式PCR技术检测98例早期自然流产胎盘绒毛组织B19的表达情况。结果 98例早期自然流产胎盘绒毛组织中,常规HE染色发现4例绒毛血管中个别有核红细胞的细胞核内出现特征性B19病毒包涵体,免疫组化有6例B19 VP1/VP2蛋白表达阳性,巢式PCR分析有8例出现B19 DNA阳性条带。结果显示98例自然流产胎盘绒毛组织中共有8例检出B19病毒,总检出率为8.2%(8/98)。结论早期自然流产胎盘绒毛组织中的B19检出率较低,B19感染可能是只是导致早期自然流产的原因之一。
Objective To investigate the relationship between B19 infection and early spontaneous abortion by detecting human parvovirus B19 (B19) in placental villi of early spontaneous abortion, using histology, immunohistochemistry and nested polymerase chain reaction (PCR). Methods The expression of B19 in placenta villi of 98 cases of early spontaneous abortion was detected by routine HE staining, immunohistochemistry and nested PCR. Results 98 cases of early spontaneous abortion placental villi, routine HE staining found in 4 villous blood vessels in the nuclei of individual nucleated erythrocytes characteristic B19 virus inclusion bodies, immunohistochemical positive expression of B19 VP1 / VP2 protein, nest There were 8 cases of positive B19 DNA bands by PCR analysis. The results showed that a total of 8 out of 98 cases of spontaneous abortion placental villi were detected B19 virus, the total detection rate was 8.2% (8/98). Conclusions The detection rate of B19 in placenta villi of early spontaneous abortion is low, and B19 infection may only be one of the causes of early spontaneous abortion.