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目的观察大黄酸(Rhein)对高糖培养大鼠近端肾小管上皮细胞肥大影响。方法无菌分离SD大鼠单根近球小管并培养;高糖(30 mM)培养肾小管上皮细胞,加入30,15,5 mg/L大黄酸,作用72 h,检测细胞体积、3H-亮氨酸掺入量及蛋白质含量等细胞肥大指标。结果高糖培养72 h导致细胞体积明显增大,3H-亮氨酸掺入量及细胞内蛋白质含量分别为(16 225.0±1 476.1)cpm/105细胞和(4.43±0.38)μg/105细胞,明显增加;30 mg/L大黄酸处理72 h后,3H-亮氨酸掺入量及细胞内蛋白质含量分别为(10 734.0±978.9)cpm/105细胞和(4.04±0.23)μg/105细胞,大黄酸可明显抑剂高糖所致细胞体积增大、3H-亮氨酸掺入量及细胞内蛋白质含量的升高。结论大黄酸可抑制高糖诱导的肾小管上皮细胞肥大。
Objective To observe the effects of Rhein on the hypertrophy of proximal tubular epithelial cells in high glucose cultured rats. METHODS: Single tube of SD rat was aseptically isolated and cultured; high glucose (30 mM) cultured renal tubular epithelial cells were incubated with 30, 15 and 5 mg/L rhein for 72 h to detect cell volume and 3H-light. Protein hypertrophy indicators such as incorporation and protein content. Results The high-glucose culture for 72 h resulted in a significant increase in cell volume. The 3H-leucine incorporation and intracellular protein content were (16 225.0±1 476.1) cpm/105 cells and (4.43±0.38) μg/105 cells, respectively. Significantly increased; 3H-leucine incorporation and intracellular protein content were (10 734.0±978.9) cpm/105 cells and (4.04±0.23) μg/105 cells after treatment with 30 mg/L rhein for 72 h. Rhein can obviously inhibit the increase of cell volume, 3H-leucine incorporation and intracellular protein content caused by high glucose. Conclusion Rhein can inhibit hyperglycemia-induced renal tubular epithelial cell hypertrophy.