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目的建立固相萃取-高效液相色谱法同时测定饮料中10种食品添加剂苯甲酸、山梨酸、糖精钠、柠檬黄、苋菜红、胭脂红、日落黄、赤藓红、亮蓝、诱惑红的方法。方法样品经固相萃取后,经色谱柱Waters sunfire C18(4.6 mm×250 mm,5μm)进行分离,流动相为甲醇-乙酸铵溶液进行梯度洗脱,柱温为30℃;进样量为10μl,流速为1.0 ml/min,检测波长为230 nm。结果 10种食品添加剂40 min内完全分离,最低检出限为0.08 mg/L~0.24 mg/L,在3个浓度水平下,加标回收率为87.8%~103.9%。相对标准偏差(RSD)为0.94%~5.41%。结论该方法简单、准确可靠,适用于饮料中10种食品添加剂的同时测定。
OBJECTIVE To establish a solid phase extraction and high performance liquid chromatography (HPLC) simultaneous determination of 10 kinds of food additives benzoic acid, sorbic acid, sodium saccharin, lemon yellow, amaranth, carmine, sunset yellow, erythrosine, method. Methods The samples were separated by solid-phase extraction on a Waters sunfire C18 column (4.6 mm × 250 mm, 5 μm). The mobile phase consisted of methanol-ammonium acetate gradient and the column temperature was 30 ℃. The injection volume was 10 μl , Flow rate of 1.0 ml / min, detection wavelength of 230 nm. Results The results showed that 10 kinds of food additives were completely separated within 40 min with the detection limit of 0.08 mg / L ~ 0.24 mg / L. The spiked recoveries ranged from 87.8% to 103.9% at the three concentration levels. The relative standard deviations (RSDs) ranged from 0.94% to 5.41%. Conclusion The method is simple, accurate and reliable and suitable for the simultaneous determination of 10 kinds of food additives in beverages.