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目的:探讨间充质干细胞对颞下关节炎患者血清PRG4及TGF-β1的软骨生成作用。方法:选取我院颞下颌关节炎患者72例,随机分为实验组和对照组。对照组予盐酸氨基葡萄糖片口服1个月,玻璃酸钠注射液颞下颌关节腔每周1次,注射4次治疗。实验组予间充质干细胞培养、自体移植,生理盐水0.5 m L稀释细胞5×105个关节腔内注射,3天后再次相同剂量及方式注射。两组患者分别测定血清TGF-β1、IL-1α和PRG4蛋白表达,最大张口度测试及疼痛程度评分,并通过影像学检查,评估两组患者关节组织损伤修复时间。结果:1与对照组相比实验组TGF-β1和PRG4蛋白表达上升更迅速且维持在较高水平,IL-1α下降更显著,差异有统计学意义(P<0.05);2与对照组比较,实验组最大张口度增加、张口时疼痛评分下降,差异有统计学意义(P<0.05);3与对照组比较,实验组受损关节组织均修复时间更短,差异有统计学意义(P<0.05)。结论:间充质干细胞可通过调节血清TGF-β1、IL-1α水平和PRG4蛋白表达,促进软骨的生成,对改善预后,提高患者生活质量有重要意义。
Objective: To investigate the effect of mesenchymal stem cells on cartilage formation in serum of patients with sub-temporal arthritis and PRG4 and TGF-β1. Methods: 72 cases of temporomandibular joint arthritis in our hospital were randomly divided into experimental group and control group. The control group was treated with glucosamine hydrochloride tablets orally for 1 month, sodium hyaluronate injection temporomandibular joint cavity once a week and 4 times for injection. In the experimental group, mesenchymal stem cells were cultured, autologous transplantation, 0.5 × L normal saline diluted 5 × 105 intra-articular injection, 3 days after the same dose and manner of injection. Two groups of patients were measured serum TGF-β1, IL-1α and PRG4 protein expression, maximum mouth opening test and pain score, and by imaging examination, to assess the two groups of patients with joint tissue injury repair time. Results: 1 Compared with the control group, the expression of TGF-β1 and PRG4 in the experimental group increased more rapidly and maintained at a higher level, the IL-1α decreased more significantly, the difference was statistically significant (P <0.05); 2 Compared with the control group (P <0.05) .3 Compared with the control group, the repair time of the damaged joint tissue in the experimental group was shorter, the difference was statistically significant (P <0.05) <0.05). Conclusion: Mesenchymal stem cells can promote the production of cartilage by regulating the levels of serum TGF-β1, IL-1α and PRG4 protein, which is of great significance for improving the prognosis and improving the quality of life of patients.