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对西伯利亚立克次体中国分离株W—88(人株),JH—74(草原革蜱株),TO—88(草原革蜱蜱卵株),BJ—90(中华革蜱株)及国际标准株(西伯利亚立克次体246株)进行扫描电镜和超薄切片免疫电镜观察。结果显示中国株与国际株具有相似的外部形态和内部超微结构。可见到立克次体细胞外表的粘液层和微荚膜;细胞包膜的细胞壁和胞浆膜;胞内结构的DNA丝和核糖体。在固定液中加入相应的免疫血清用钌红细胞化学染色,在立克次体表面可见有肥厚的纤维状物(亚晶格状表面层)围绕的粘液层,经乙醚提取的立克次体标本粘液层消失,而用泛影(?)胺密度梯度离心纯化的立克次体标本仍有粘液层存在,此为进一步研究其化学成分和功能提供了必要条件。
The results showed that the resistance of Rickettsia sibirica W-88 (human strain), JH-74 (strain of turf grass), TO-88 (tick grasshopper tick), BJ-90 The standard strain (246 strains of Rickettsia sibirica) was observed by scanning electron microscopy and ultrathin section immunoelectron microscopy. The results show that Chinese strains and international strains have similar external morphology and internal ultrastructure. Visible to the rickettsial cell surface mucus layer and micro-capsule; cell envelope cell membrane and cytoplasmic membrane; intracellular structure of the DNA filaments and ribosomes. In the fixed solution by adding the corresponding immune serum with ruthenium red blood cells chemical staining in the rickettsia surface can be seen with hypertrophic fibrous material (sub-lattice-like surface layer) around the mucus layer, the ether extract of Rickettsia specimens The mucus layer disappeared. However, there was still a mucus layer in the rickettsia samples which were centrifuged with universal gradient (?) Amine density gradient, which provided the necessary conditions for further study of its chemical composition and function.