论文部分内容阅读
目的研究α-突触核蛋白(α-Synuclein,α-SYN)是否存在于正常大鼠脑线粒体中以及是否能够转运至线粒体内。方法用免疫印迹和免疫电镜方法检测大鼠脑线粒体中是否存在α-SYN。将人重组α-SYN与分离的线粒体孵育,用免疫印迹法检测α-SYN是否可以转运到线粒体中。结果免疫印迹和免疫电镜方法均证实α-SYN广泛存在于正常大鼠脑的线粒体中,不同脑区的α-SYN含量不同,其中纹状体、嗅球、海马和丘脑的线粒体中α-SYN的含量高于大脑皮质、脑干和小脑。人重组α-SYN与不含内源性α-SYN的分离大鼠小脑线粒体进行孵育,表明α-SYN以剂量依赖的方式转移至线粒体内。结论在正常情况下α-SYN存在于线粒体内,线粒体的α-SYN有可能源于胞质α-SYN向线粒体的转运。
Objective To investigate whether α-synuclein (α-SYN) exists in normal rat brain mitochondria and whether it can transport to α-synuclein. Methods Western blotting and immunoelectron microscopy were used to detect the presence of α-SYN in rat brain mitochondria. Human recombinant α-SYN was incubated with isolated mitochondria and Western blotting was used to detect whether α-SYN can be transported to mitochondria. Results Western blotting and immunoelectron microscopy confirmed that α-SYN exists widely in the mitochondria of normal rat brain. The content of α-SYN in different brain regions is different. The mitochondria α-SYN in the striatum, olfactory bulb, hippocampus and thalamus Higher than the cerebral cortex, brain stem and cerebellum. Human recombinant α-SYN was incubated with isolated rat cerebellar mitochondria without endogenous α-SYN, indicating that α-SYN is transferred to mitochondria in a dose-dependent manner. Conclusions α-SYN exists in the mitochondria under normal conditions. Mitochondrial α-SYN may be due to the transport of cytoplasmic α-SYN to mitochondria.