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目的 观察高血压大鼠视网膜色素上皮 (retinal pigment epithelium,RPE)细胞超微结构的改变及其屏障功能受损情况 ,探讨其与神经视网膜病变的关系。 方法 用胶体镧示踪结合透射电镜观察5、6、7个月龄自发性高血压大鼠 RPE细胞超微结构改变及脉络膜视网膜屏障渗透性的改变 ,并与同月龄正常京都种大鼠作对照。 结果 1高血压大鼠 RPE细胞中线粒体肿胀 ,内质网扩张 ,基底部皱褶减少 ,微绒毛变稀疏。这些退行性改变随鼠龄增大与血压的增高而加重。 2 6、7个月龄大鼠脉络膜视网膜屏障破坏 ,脉络膜毛细血管中的胶体镧进入到 RPE细胞内褶及细胞间隙 ,且能通过紧密连接到达细胞顶部进入视网膜下间隙 ,而 5个月龄高血压大鼠及各种正常大鼠、胶体镧不能通过此屏障 ,仅到达 RPE细胞之间。 结论 高血压大鼠视网膜病变的早期 RPE细胞已有缺血、缺氧所致的退行性改变 ,且伴有脉络膜视网膜屏障的渗透性增加
Objective To observe the changes of the ultrastructure of retinal pigment epithelium (RPE) cells and the impaired barrier function in hypertensive rats, and to explore its relationship with neuroretinopathy. Methods The ultrastructural changes of RPE cells and the barrier permeability of choroidal membrane in spontaneous hypertensive rats of 5,6,7 months old were observed by colloidal lanthanum tracing combined with transmission electron microscopy and compared with that of normal Kyoto rats of the same age . Results 1 Hypertensive rat RPE cells mitochondria swelling, endoplasmic reticulum dilatation, basal fold reduction, microvilli thinning. These degenerative changes with increasing age and increased blood pressure and aggravate. The choroidal retina barrier was damaged in rats of 6 and 7 months old. Colloidal lanthanum in choroidal capillaries entered into the pleated and intercellular space of RPE cells and reached the subretinal space through tight junction to reach the top of the cells, while 5-month-old Blood pressure rats and various normal rats, colloidal lanthanum can not pass this barrier, only reached between RPE cells. Conclusion The early RPE cells of retinopathy of hypertensive rats have been ischemia, hypoxia-induced degenerative changes, and associated with increased permeability of the chorioretinal barrier