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将大鼠大脑皮层勻浆经离心分离的大突触体组份同含一定量的川棟素溶液于生理条件(温度、pH、离子强度)下保温,再经离心分离后,用对二甲氨基苯甲醛试剂显色的分光光度法测定上清液中的川棟素含量,结果表明上清液中川棟素含量明显降低,在一定范围内,降低的程度随大突触体组分浓度的提高而增加。而同样处理的肝组织、脑白质与川棟素保温后,上清液中川棟素含量基本不变。由这些结果看来,可能川棟素同突触体有一选择性的结合。
The synaptosomal fraction of rat cerebral cortex homogenized by centrifugation was incubated with a certain amount of chuanodong solution under physiological conditions (temperature, pH, ionic strength) and centrifuged, Aminobenzaldehyde reagent spectrophotometric determination of the content of Chuan Dong Su in the supernatant, the results showed that the content of Chuan Dong Su significantly decreased in a certain range, the degree of reduction with the concentration of synaptic components Increase and increase. The same treatment of liver tissue, white matter and Chuan-Dong insulation, the supernatant content of Chuan-Dong basically unchanged. From these results, it seems likely that Chuan Dong Su with a selective combination of synaptosomes.