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目的 建立稳定、价廉、实用的慢性萎缩性胃炎动物模型。 方法 采用正交试验 ,以60%乙醇加20mmol/L去氧胆酸钠、0.1%氨水、0.5mg/ml吲哚美辛3因素刺激SD大鼠胃粘膜24周。 结果 3因素联合作用胃窦部和胃体部炎症级别 (2.50和1.71)分别较空白对照组 (0.50和0.75)高 ,胃窦部固有层厚度 (2.17μm)和固有腺面积百分比 (17.5% )分别比空白对照组 (105μm和65.8% )低 ,胃体部固有层厚度与空白对照组差别无显著性意义 ,壁细胞数 (21)较空白对照组 (30.6)减少。停止作用5周后病变程度无改变。 结论 3因素联合作用24周能建立典型的鼠慢性萎缩性胃炎模型 ,且稳定性好。
Objective To establish a stable, inexpensive and practical animal models of chronic atrophic gastritis. Methods Orthogonal test was used to stimulate SD rat gastric mucosa for 24 weeks with 60% ethanol plus 20 mmol / L sodium deoxycholate, 0.1% aqueous ammonia and 0.5 mg / ml indomethacin 3. Results The combination of three factors (2.50 and 1.71) in gastric antrum and gastric body were significantly higher than that in control group (0.50 and 0.75), the thickness of lamina propria (2.17μm) and the percentage of intrinsic glandular area (17.5% (105μm and 65.8%) respectively. There was no significant difference in the lamina propria of gastric body and blank control group, and the number of parietal cells (21) was lower than that of blank control group (30.6). No effect on the lesion degree after 5 weeks of stopping. Conclusions The combination of three factors can establish a typical model of chronic atrophic gastritis in rats for 24 weeks, and its stability is good.