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目的探讨凝血酶对内皮细胞组织因子（ＴＦ）活性的刺激作用及其与细胞内蛋白激酶Ｃ（ＰＫＣ）传导系统的关系。方法传代培养新生牛主动脉血管内皮细胞，取刺激或未受刺激的第４～８代细胞冻融液测定其ＴＦ活性。结果体外培养的血管内皮细胞在静息状态下ＴＦ活性极低（４．２０±１．１９）。与对照组相比，凝血酶的刺激使内皮细胞ＴＦ活性明显升高（ｎ＝８，Ｐ＜０．００１），且呈剂量依赖性关系（ｒ＝０．７８，Ｐ＜０．０５）和时间依赖性关系（ｒ＝０．８８，Ｐ＜０．０５）。ＰＫＣ抑制剂Ｈ７抑制凝血酶对内皮细胞ＴＦ活性的刺激作用（ｎ＝８，Ｐ＜０．０１），且抑制作用呈剂量依赖性。ＰＫＣ激动剂佛波酯也可刺激内皮细胞ＴＦ活性增高（３０．５９±３．７９，ｎ＝８，Ｐ＜０．００１），并可被Ｈ７阻断，ＰＭＡ对凝血酶的刺激作用没有显著影响。结论在凝血酶的刺激下，血管内皮细胞可产生较大量的ＴＦ；凝血酶对内皮细胞的这种刺激作用依赖于细胞内ＰＫＣ系统传导途径。
Objective To investigate the stimulatory effect of thrombin on endothelial cell factor (TF) activity and its relationship with intracellular protein kinase C (PKC) conduction system. Methods Newborn bovine aortic endothelial cells were subcultured, and the TF activity was measured by frozen and thawed cells from the 4th to 8th generation cells. Results The endothelial cells cultured in vitro showed very low TF activity (4.20 ± 1.19) at rest. Compared with the control group, thrombin stimulation significantly increased the activity of TF in endothelial cells (n = 8, P <0.001) and in a dose-dependent manner (r = 0.78, P <0.05) The time-dependent relationship (r = 0.88, P <0.05). PKC inhibitor H7 inhibited the stimulating effect of thrombin on TF activity of endothelial cells (n = 8, P <0.01), and the inhibitory effect was dose-dependent. PKC agonist phorbol ester also stimulated endothelial cell TF activity increased (30.59 ± 3.79, n = 8, P <0.001), and was blocked by H7, PMA stimulation of thrombin was not significant influences. Conclusions Thrombin stimulates vascular endothelial cells to produce larger amounts of TF; this stimulation of endothelial cells by thrombin relies on intracellular PKC system transduction pathways.