论文部分内容阅读
芥蓝(Brassica oleracea L.var.alboglabra Bailey)是起源于我国南方的甘蓝性蔬菜。为探讨和评价芥蓝种质资源的遗传多样性,本研究选用15个典型的芥蓝品种进行了7个性状的表型多样性和SCo T多态性分析。结果发现齐口期、株高、茎粗、开展度、叶重、薹茎重和分枝数的变异系数分别为21.60%、30.32%、23.21%、69.77%、65.16%、48.71%和43.04%。只有薹茎重与叶重的线性相关达到显著水平。表型分析的遗传距离变化范围是22.865 5~269.316 5,平均值是113.817 3,在122.7处将15个品种分为3类。SCo T分析中29条引物扩增出119条带,平均每条引物4.1条带,多态性条带93条,多态性百分率为78%。在各个品种中平均扩增79条。分子遗传距离变化范围为0.154 2~0.516 4,平均值是0.339 9,在0.20处将15个品种分为3类。10个品种的表型和分子聚类结果相同,但mantel test检验表明表型多样性和SCo T多态性相关不显著。本研究结果表明SCo T可以作为一种鉴定芥蓝种质资源遗传多样性的简单的常规分子标记。
Kale (Brassica oleracea L.var.alboglabra Bailey) is originated in southern China cabbage vegetables. In order to explore and evaluate the genetic diversity of kale germplasm resources, phenotypic diversity and SCo T polymorphism of seven traits were analyzed in 15 typical Chinese kale cultivars. The results showed that the coefficient of variation (CV) was 21.60%, 30.32%, 23.21%, 69.77%, 65.16%, 48.71% and 43.04% at Qi mouth stage, plant height, stem diameter, . Only the linear correlation between stem weight and leaf weight reached a significant level. The genetic distance ranged from 22.865 5 to 269.316 5 for phenotypic analysis, with an average of 113.817 3, and 15 varieties were divided into 3 categories at 122.7. In the SCo T analysis, 119 bands were amplified by 29 primers, with an average of 4.1 bands per primer and 93 polymorphic bands with a percentage of polymorphism of 78%. In each species an average of 79 amplification. The range of molecular genetic distance varied from 0.154 2 to 0.516 4 with an average value of 0.339 9, and 15 varieties were divided into 3 categories at 0.20. The 10 cultivars had the same phenotype and molecular clustering results, but mantel test showed no significant correlation between phenotypic diversity and SCo T polymorphism. The results of this study indicate that SCo T can be used as a simple conventional molecular marker to identify the genetic diversity of kale germplasm.