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目的探讨放血治疗足底电击致大鼠焦虑样行为的影响及其作用机制。方法将23只健康雄性SD大鼠分为正常组(7只)和造模组(16只)。造模组采用足底电击结合孤养的方法制备焦虑样模型大鼠,正常组不干预;将造模成功的14只大鼠按行为学评分随机分为模型组与放血组,放血组尾静脉放血(15滴,1次),模型组不干预。治疗第7天高架十字迷宫实验评价焦虑样行为,逆转录聚合酶链反应RT-PCR方法和免疫组织化学方法分别检测各组大鼠海马内皮源型一氧化氮合酶(e NOS)mRNA和CA1区蛋白的表达结果。结果大鼠接受足底电击后在开臂/(开臂+闭臂)的时间百分比和次数百分比显著降低(P<0.01),放血升高(P<0.01),升高幅度与正常组相当(P>0.05)。足底电击刺激大鼠后海马内e NOS mRNA和CA1区e NOS蛋白表达降低(P<0.05),放血反之(P<0.05),上调结果与正常组无差异(P>0.05)。结论放血可以改善焦虑样行为,可能是通过调节海马内e NOS起作用的。
Objective To investigate the effect and mechanism of blood-letting treatment on anxiety-like behavior of rats after foot electric shock. Methods Twenty-three healthy male SD rats were divided into normal group (n = 7) and model group (n = 16). The rats in model group were prepared by foot shock combined with solitary method, and the rats in normal group were not intervened. The 14 rats with successful model were randomly divided into model group and bleed group according to behavioral score, Bleeding (15 drops, 1 time), the model group did not interfere. The anxiety-like behavior was assessed by the elevated plus-maze test on the 7th day. The eNOS mRNA and CA1 were detected by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry District protein expression results. Results The percentage of percentage and frequency of opening arm / (open arm + closed arm) were significantly decreased (P <0.01) and the blood was exsanguinated (P <0.01) in rats after foot shock, P> 0.05). The hippocampal eNOS mRNA and eNOS protein expression in hippocampus were decreased (P <0.05) and the blood was excreted in the foot shock (P <0.05). The upregulation results were not significantly different from the normal group (P> 0.05). Conclusion Bloodletting can improve anxiety-like behavior, possibly by regulating eNOS in hippocampus.