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自从Sutherland于1948年提出血浆中的高血糖素可能有非胰腺的来源后,人们就一直在寻找究竟还有哪些器官和组织可能产生高血糖素。继在胃肠道和下颌腺发现高血糖素免疫反应性物质后,最近日本Osaka大学的Tanaka等又报告,在大鼠的血管壁有高血糖素的免疫反应物质。他们采用两种高血糖素的抗血清,用免疫组织化学和免疫化学方法进行观察。其中抗血清OAL-123,是用牛胰高血糖素C端19-29片段免疫兔得到的,它与肠高血糖素的交叉反应小于0.1%,与其它胃肠肽类如生长抑素、P物质、神经降压素、脑啡肽、胆囊收缩素等都无交叉反应。另一种抗血清30K,是被广泛应用的与胰高血糖素C端起反应的抗血清。
Since Sutherland proposed in 1948 that plasma glucagon may have a non-pancreatic origin, people are constantly looking for which organs and tissues are likely to produce glucagon. Following the discovery of glucagon-like immunoreactive substances in the gastrointestinal tract and mandibular glands, Tanaka et al. Recently reported that glucagon-like immunoreactive substances are present in the blood vessel wall of rats. They used two anti-glucagon antisera and observed them by immunohistochemistry and immunochemistry. Among them, antiserum OAL-123 was obtained by immunizing rabbits with the C-terminal 19-29 fragment of bovine glucagon and its cross-reactivity with glucagon was less than 0.1%. Compared with other gastrointestinal peptides such as somatostatin, P Substance, neurotensin, enkephalin, cholecystokinin and so no cross-reaction. Another antiserum, 30K, is a widely used antiserum that responds to the C-terminal glucagon.