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1 材料与方法1 1 质粒的构建ｐＣＰ10质粒含有ａｙｗ亚型ＨＢＶ全基因组。该质粒作为扩增Ｓ蛋白基因的模板 ,上、下游引物含ＢｇｌⅡ的酶切位点。Ｐ1:5′ＧＧＡＡＧＡＴＣＴＡＴＧＧＡＧＡＡＣＡＴＣＡＣＡＴＣＡＧＧ 3′,Ｐ2 :ＧＧＡＡＧＡＴＣＴＴＡＡＡＴＧＴＡＴＡＣＣＣ
1 Materials and methods 1 1 plasmid construction pCP10 plasmid contains ayw subtype HBV whole genome. The plasmid as a template for amplification of S protein gene, upstream and downstream primers containing Bgl Ⅱ restriction sites. P1: 5’GGAAGATCTATGGAGAACATCACATCAGG 3 ’, P2: GGAAGATCTTAAATGTATACCC