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目的 :分离和鉴定我国流行的人免疫缺陷病毒 (humanimmunodeficiencyvirus ,HIV)毒株 ,研究我国HIV毒株的生物学特性。方法 :用外周血单个核细胞 (peripheralbloodmononuclearcells ,PBMC)共培养的方法分离HIV ,用观察细胞病变、检测P2 4抗原和前病毒DNA的方法监测病毒生长的情况。结果 :在建立共培养的第 12天观察到明显的细胞病变 ,主要表现为大泡样细胞和多核巨细胞。接种第 9天细胞培养上清液的P2 4抗原由阴性转为阳性 ,并且浓度迅速增加 ,接种第 12天浓度即达到 1780pg/ml ,第 14天达到峰值 ,为 2 32 0pg/ml。但该株病毒的PBMC培养上清液转种于MT4细胞后 ,培养上清液P2 4抗原和前病毒DNA始终为阴性且不出现细胞病变。结论 :用PBMC共培养的方法从一名河北籍HIV感染者的PBMC分离出了人免疫缺陷病毒HB J株 ,它是快速生长的、嗜巨噬细胞的、致多核巨细胞形成的毒株 ,而且只感染新鲜的人PBMC而不感染传代人T淋巴细胞MT4 ,可能与使用的辅助受体有关 ,值得深入研究。
OBJECTIVE: To isolate and identify the human immunodeficiency virus (HIV) strains that are endemic in China and to study the biological characteristics of HIV strains in our country. Methods: Human peripheral blood mononuclear cells (peripheral blood mononuclear cells, PBMC) were co-cultured to isolate HIV, and the growth of the virus was monitored by observing cytopathic effect, detecting P2 4 antigen and provirus DNA. RESULTS: Significant cytopathic changes were observed on day 12 of establishing co-culture, mainly manifested as lamellar cells and multinucleated giant cells. The P2 4 antigen in the cell culture supernatant on the 9th day of inoculation turned negative from positive to positive, and the concentration rapidly increased to 1780 pg / ml on the 12th day of inoculation and reached 2 32 0 pg / ml on the 14th day. However, after the PBMC culture supernatant of this strain of virus was switched to MT4 cells, the culture supernatant P2 4 antigen and proviral DNA were always negative and cytopathic effect did not occur. CONCLUSION: Human immunodeficiency virus HB strain J is isolated from the PBMC of HIV-infected persons in Hebei province by means of PBMC co-culture. It is a rapidly growing, macrophage-producing and multinucleate giant cell-forming strain, And only infected with fresh human PBMC without infection of passaged T lymphocyte MT4 may be related to the use of co-receptors, it is worth further study.