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不少报导已证明,促黄体生成素释放激素(LH-RH)及其高活性类似物在几种动物具有抗生育活性。本工作在大鼠观察了LH-RH对卵巢黄体生成素(LH)受体水平的影响,以期阐明其抗生育作用的机理。作者在大鼠妊娠的第一至七天(着床前及着床期)或第七至十二天(着床后期),每天每鼠皮下注射200微克LH-RH,对照动物注射等量油溶剂。隔天处死一批动物,检查着床点数,测定卵巢对LH的结合,并取血测定血清黄体生成素(LH),卵泡刺激素(FSH),生乳素,雌二醇及孕酮。结果表明,LH-RH明显地降低正常着床点数,单次注射LH-RH后24小时内,卵巢LH结合即显著减少,并于用药过程中持续减少。经分析,这一LH结合的减少系因卵巢LH受体的浓度减少,而不是由于受体平衡常数改变。连续用LH-RH7
Many reports have shown that luteinizing hormone-releasing hormone (LH-RH) and its highly active analogs have anti-fertility activity in several animals. This work in rats observed LH-RH ovarian luteinizing hormone (LH) receptor levels, in order to clarify its anti-fertility mechanism. The authors subcutaneously injected 200 μg of LH-RH per mouse per day on the first to seventh days (pre-implantation and implantation) or on the seventh to twelfth days (late implantation) of pregnant rats. Control animals were injected an equal volume of oil solvent . A number of animals were sacrificed on the next day. The number of implantation sites was examined. The ovaries were determined for their binding to LH. Blood samples were taken for serum LH, FSH, lactogen, estradiol and progesterone. The results showed that, LH-RH significantly reduced the normal implantation point, a single injection of LH-RH within 24 hours after the ovarian LH binding was significantly reduced, and continued to decrease in the course of medication. This decrease in LH binding was analyzed due to reduced concentrations of ovarian LH receptors rather than changes in receptor balance constants. Continuous use LH-RH7