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宽翅菘蓝(Isatis violascens)是分布于新疆准噶尔盆地的一种早春短命植物。基于形态的相似性,有人建议将宽翅菘蓝作为Isatis emarginata同义体。目前宽翅菘蓝尚未得到分子水平的研究,其系统发育地位也不清楚。该研究对宽翅菘蓝的ITS区进行测序,结合GenBank数据库中菘蓝属物种的ITS序列对宽翅菘蓝的系统关系和分类地位进行研究。最大简约法,最大似然法和贝叶斯法3种方法分析表明:菘蓝属物种聚为两个分支;宽翅菘蓝与I.emarginata和小果菘蓝具有较近的亲缘关系;基于形态相似性和较近的遗传距离,支持将宽翅菘蓝与I.emarginata合并为一个物种,并把宽翅菘蓝作为I.emarginata的同义种。
Isatis violascens is a kind of early spring ephemeral plants distributed in the Junggar basin of Xinjiang. Based on morphological similarities, it is suggested that Isatis emarginata is a synonym of Isatis emarginata. At present, there is no research on the molecular level of Polyphaga solani, and its phylogenetic position is also unclear. In this study, the ITS region of Isatis indigotica was sequenced. The phylogenetic relationships and taxonomic status of Isochrysis plutellae were studied based on ITS sequences of the genus Brachydine in the GenBank database. Maximum parsimony method, maximum likelihood method and Bayesian method analysis showed that: Polygonum species are clustered into two branches; Broad-winged raccoon dog Blue and I.emarginata have a relatively close relationship; based on Morphological similarities and closer genetic distances supported the merging of Broad-winged 菘 blue with I.emarginata into one species and the use of 翅 菘 菘-blue as a synonym for I.emarginata.