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虫生轮枝孢(Verticillium insectorum)寄生于南方根结线虫(Meloidogyne incognita)的雌虫和四龄幼虫,此菌在浙江省棉花和黄杨根结线虫上的自然寄生率分别为10.2％和20.5％,是棉花根结线虫天敌真菌区系中的优势种,占77.3％。在25—30℃范围内生长,产孢和孢子萌发均好;当外界相对湿度达到75％时,此菌开始生长并产孢,但孢子萌发需要较高的相对湿度(95％)。基质pH值的变化对产孢影响不大,在pH5—9范围内,生长均好,但孢子萌发需较严格的pH值,以pH7为最好。此菌在马铃薯琼脂培养基和玉米琼脂培养基上生长较快,查彼克培养基对产孢最有利。V.insectonum对棉花的生长发育无不良影响,在盆栽试验中,用此菌(10~6个孢子/1克土)和南方根结线虫卵(2500粒/盆)接种棉花根围,有71.0—89.1％的雌虫被寄生。
The parasitism rates of the parasitism of the virulent strains of Verticillium insectorum on the female and fourth instar larvae of Meloidogyne incognita in Zhejiang province were 10.2% and 20.5%, respectively. , Is the dominant species in the fungal flora of cotton root knot nematode, accounting for 77.3%. The spores germinated well in the range of 25-30 ℃. When the relative humidity reached 75%, the spores began to grow and sporulation, but the spore germination required higher relative humidity (95%). The change of substrate pH had little effect on sporulation, and the growth was good in the range of pH5-9. However, the germination of sporophytes needed a more stringent pH value, and pH7 was the best. This strain grows faster on potato agar and corn agar, and Chapirk medium is most favorable for sporulation. V.insectonum had no adverse effects on the growth and development of cotton. In the pot experiment, cotton rhizosphere was inoculated with the fungus (10-6 spores / 1 gram of soil) and M. elegans eggs (2500 grains / pot) 71.0-89.1% of females are parasitic.