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目的:研究镰形棘豆总生物碱的体内抗肿瘤作用并探讨其作用机制。方法:采用S180荷瘤小鼠模型,将生理盐水和总生物碱灌胃给药,阳性药环磷酰胺腹腔注射,给药后检测小鼠的体质量、抑瘤率、胸腺指数以及脾指数,并通过HE染色,光镜下观察肿瘤细胞的形态变化。结果:镰形棘豆总生物碱高、中、低剂量组对S180荷瘤小鼠的抑瘤率分别为74.8%、79.3%、69.4%,总生物碱各剂量组对S180荷瘤小鼠的胸腺指数和脾指数均高于环磷酰胺组和生理盐水组,但无显著性差异。实验过程中,小鼠体质量没有降低,未显示任何毒性反应。HE染色发现,给药组肿瘤间质可见炎细胞浸润。结论:镰形棘豆总生物碱具有明显的抗肿瘤作用,其机制可能是通过增强小鼠的免疫调节功能来实现的。
Objective: To study the antitumor effect of total alkaloids from Oxytropis falcate and to explore its mechanism. Methods: S180 tumor-bearing mice model was established by intragastric administration of saline and total alkaloids. The mice were injected intraperitoneally with cyclophosphamide, and the body weight, tumor inhibition rate, thymus index, spleen index, The morphological changes of tumor cells were observed under light microscope by HE staining. Results: The antitumor rates of Salk alkaloids in S180 tumor-bearing mice were 74.8%, 79.3% and 69.4%, respectively. The inhibitory rates of total alkaloids in S180 tumor-bearing mice Thymus index and spleen index were higher than cyclophosphamide group and saline group, but no significant difference. During the experiment, the body weight of mice did not decrease and did not show any toxic reaction. Hematoxylin-eosin staining found that infiltration of inflammatory cells in the tumor stroma of the treated group was observed. Conclusion: The total alkaloids of Oxytropis falcate have obvious anti-tumor effects, and its mechanism may be through enhancing the immunomodulatory function of mice.