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利用散布性重复序列(dispersedrepetitivesequence)MGR586探针与EcoRI组合,分析了采自广东省4个自然生态稻作区的112个猪瘟病菌株的限制性片断长度多态性(RFLPs),根据彼此间RFLPs单型(haplotype)的带型位置相似率达80%为度,把这些菌株划分为15个遗传宗谱(geneticlineage),其中宗谱1及宗谱2占总数的78.58%,遍布全省各地,是优势宗谱。供测菌株的RFLPs单型的多样性显示出我省稻瘟病菌的群体结构呈现多样性。参试菌株的群体遗传多样性值为0.64。谱型分析表明,我省多数病原型是杂合群体。在分子水平上划分的病菌宗谱与寄生品种的遗传背景有密切关系。研究结果也初步建立了病菌宗谱与用以鉴别寄主反应划分的生理小种致病谱型的相互关系。
The restriction fragment length polymorphism (RFLPs) of 112 M. grisea strains collected from four natural ecological rice paddies in Guangdong Province were analyzed by using the dispersed repetitive sequence MGR586 probe in combination with EcoRI. RFLPs with a haplotype banding of 80% similarity were classified into 15 genetic lineages, of which genealogical 1 and genealogy 2 accounted for 78.58% of the total Provinces, is the dominant genealogy. The single haplotype diversity of RFLPs for the tested strains showed that the population structure of M. grisea showed diversity in our province. The population genetic diversity of the tested strains was 0.64. Genealogical analysis shows that most of the pathogenic bacteria in our province are heterozygous groups. The molecular phylogeography of the bacteria is closely related to the genetic background of the parasitic species. The results also initially established the relationship between the germline genealogies and the pathogenic patterns of the races used to identify the host response.