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色谱技术中的化学衍生法系指在色谱过程中用特殊的化学试剂(一般称为衍生化试剂或标记试剂)使样品成分转变相应的衍生物之后进行分离检测或进行检测的方法。近十年来,化学衍生法在高效液相色谱法中的应用受到重视,发展较快。在高效液相色谱法中,化学衍生化的目的为:1.将紫外一可见强吸收功能基团引入被检测对象或将其转变为荧光衍生物,以提高检测灵敏度;2.提高对分析样品的分离和选择性。
Chromatography chemical derivatization method refers to the chromatographic process with a special chemical reagent (commonly known as derivatizing reagents or labeling reagents) so that the sample components after the corresponding derivative of the separation of detection or detection methods. In recent ten years, the application of chemical derivatization method in high performance liquid chromatography has drawn great attention and developed rapidly. In high performance liquid chromatography, the purpose of chemical derivatization is: 1. The UV-visible strong absorption functional group is introduced into the test object or converted into fluorescent derivatives to improve the detection sensitivity; 2. improve the analysis of samples Separation and selectivity.