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本文用3H TdR掺入法和MTT法观察 5HT3受体激动剂 1 phenylbiguanide(PBG )、 5HT3受体拮抗剂格拉司琼 (granisetron )和托烷司琼 (tropisetron )对体外培养ICR小鼠脾淋巴细胞ConA ,LPS刺激的增殖和NK细胞活性的影响。结果表明PBG ( 10 6～ 10 4mol/L )抑制ConA刺激的脾细胞增殖反应和脾细胞IL 2的生成 (P <0 0 5 ) ;增强LPS剌激的脾细胞增殖反应 (P <0 0 5 ) ;格拉司琼和托烷司琼双相影响 ,即在低浓度 ( 10 7～ 10 6 mol/L )促进、在较高浓度 ( 10 5～ 10 4mol/L )抑制ConA刺激的增殖反应 (P <0 0 5 ) ;二药均浓度依赖抑制LPS刺激的脾细胞增殖效应。PBG对脾淋巴细胞增殖的影响被同时加入格拉司琼或托烷司琼拮抗 ,格拉司琼和托烷司琼减弱PBG 10 5mol/L对脾细胞增殖反应的影响。本实验 5HT3受体激动剂或拮抗剂浓度不明显影响脾NK细胞活性和无丝裂原刺激的增殖反应。结果提示小鼠脾T细胞和B细胞表面可能存在对淋巴细胞增殖有不同影响的 5HT3受体
In this study, 3H TdR incorporation and MTT assay were used to observe the effects of 5HT3 receptor agonist 1 phenylbiguanide (PBG), 5HT3 receptor antagonist granisetron and tropisetron on ICR mouse splenic lymphocytes ConA, LPS stimulated proliferation and NK cell activity. The results showed that PBG (10 6 ~ 10 4 mol / L) inhibited ConA-stimulated splenocyte proliferation and splenocyte IL 2 production (P <0.05), and enhanced LPS-stimulated splenocyte proliferation (P <0.05 ); The effects of both granisetron and tropisetron on the biphasic effects were promoted at low concentrations (10 7 ~ 10 6 mol / L) and at higher concentrations (10 5 ~ 10 4 mol / L) P <0.05). Both drugs inhibited the proliferation of spleen cells stimulated by LPS in a concentration-dependent manner. The effects of PBG on splenic lymphocyte proliferation were simultaneously treated with granisetron or tropisetron, and granisetron and tropisetron attenuated the effect of PBG10 5mol / L on the splenocyte proliferation response. The experimental 5HT3 receptor agonist or antagonist concentration did not significantly affect the spleen NK cell activity and no mitogen-induced proliferative response. The results suggest that mouse spleen T cells and B cell surface may have different effects on lymphocyte proliferation 5HT3 receptor